Kim Hyung Min, Yang Gun, Kim Jung Yoon, Yoon Sang Jun, Shin Byong-Kyu, Lee Jeongmi, Park Jeong Hill, Kwon Sung Won
Seoul National University, College of Pharmacy, Seoul 08826, Korea.
Sungkyunkwan University, School of Pharmacy, Suwon 16419, Korea.
J AOAC Int. 2017 Sep 1;100(5):1492-1499. doi: 10.5740/jaoacint.17-0006. Epub 2017 Apr 18.
A simple and fast method was developed for the determination of volatile organic compounds in alcoholic beverages. Eleven volatile organic compounds (acetaldehyde, methanol, 2-propanol, tert-butanol, 1-propanol, ethyl acetate, 2-butanol, isobutanol, 1-butanol, 3-methyl-1butanol, and 2-methyl-1-butanol) in alcoholic beverages were analyzed with a simple direct-injection method using GC with flame ionization detection. These compounds should be monitored in the QC of production processes because they are detrimental to human health. The method was validated with four types of alcoholic beverages (beers, fruit wines, rice wines, and spirits) to confirm the versatility of the method. Linearity showed r2 values from 0.9986 to 0.9995, with LODs ranging from 0.010 to 1.000 mg/L. Precision and accuracy showed acceptable results, proving the effectiveness of the method. The developed method was applied to 40 commercial samples representing the four types of alcoholic beverages, and principal component analysis was performed to determine profiles of the volatile organic compounds, depending on the type of alcoholic beverage.
开发了一种简单快速的方法用于测定酒精饮料中的挥发性有机化合物。采用带火焰离子化检测的气相色谱直接进样法，分析了酒精饮料中的11种挥发性有机化合物（乙醛、甲醇、2-丙醇、叔丁醇、1-丙醇、乙酸乙酯、2-丁醇、异丁醇、1-丁醇、3-甲基-1-丁醇和2-甲基-1-丁醇）。由于这些化合物对人体健康有害，因此在生产过程的质量控制中应予以监测。该方法用四种酒精饮料（啤酒、果酒、米酒和烈性酒）进行了验证，以确认该方法的通用性。线性关系的r2值在0.9986至0.9995之间，检测限在0.010至1.000 mg/L之间。精密度和准确度结果令人满意，证明了该方法的有效性。将所开发的方法应用于40个代表四种酒精饮料类型的商业样品，并进行主成分分析以确定挥发性有机化合物的分布情况，具体取决于酒精饮料的类型。
