Liu Yisong, Liu Chunfeng, Xu Xin, Niu Chengtuo, Wang Jinjing, Zheng Feiyun, Li Qi
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Laboratory of Brewing Science and Technology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Foods. 2022 Oct 31;11(21):3450. doi: 10.3390/foods11213450.
Acetaldehyde is regarded as an important flavor compound in alcoholic beverages. With the advantages of rapidity, low cost and high sensitivity, fluorescent probe could be used as a new tool for the detection of acetaldehyde. Here, an effective fluorescence sensing method based on fluorescent probe N1 (FPN1) was established in this study. The function of FPN1 relies on the nucleophile substitution reaction and photoinduced electron transfer (PET), resulting in a fluorescence increase. Remarkably, the pretreatment background removal method (BRM) was successfully applied for removal of the interference of pyruvate and acetal. The linearity range (LR), limit of detection (LOD) and recovery of the fluorescence sensing method with BRM were 0.0053-200 mg/L, 0.0016 mg/L and 94.02-108.12%, respectively, which showed a broader detection range and better performance on sensitivity compared with the traditional quantitation using gas chromatography (GC). Furthermore, successful application of the method in real samples indicated the advantages of low-cost and rapidity for small-scale detection while assuring the accuracy, which provides a new strategy for the detection of acetaldehyde concentration in alcoholic beverages.
乙醛被认为是酒精饮料中的一种重要风味化合物。荧光探针具有快速、低成本和高灵敏度的优点，可作为检测乙醛的新工具。在此，本研究建立了一种基于荧光探针N1（FPN1）的有效荧光传感方法。FPN1的功能依赖于亲核取代反应和光致电子转移（PET），从而导致荧光增强。值得注意的是，预处理背景去除方法（BRM）成功应用于去除丙酮酸和缩醛的干扰。采用BRM的荧光传感方法的线性范围（LR）、检测限（LOD）和回收率分别为0.0053-200 mg/L、0.0016 mg/L和94.02-108.12%，与传统的气相色谱（GC）定量方法相比，具有更宽的检测范围和更高的灵敏度。此外，该方法在实际样品中的成功应用表明，在保证准确性的同时，具有低成本和快速小规模检测的优点，为酒精饮料中乙醛浓度的检测提供了一种新策略。
Zotero 插件
