Petersson F, Andersson R G, A:son Berg A, Hammar M
Department of Obstetrics and Gynaecology, University Hospital of Linköping, Sweden.
Int J Androl. 1988 Jun;11(3):179-86. doi: 10.1111/j.1365-2605.1988.tb00993.x.
Ten infertile men underwent testicular biopsy. Cyclic AMP concentration and cAMP-dependent protein kinase activity were determined in biopsies obtained before, and at 3, 10, 20 and 30 min after an intravenous injection of hCG (1500-5000 IU). The in-vitro conversion of progesterone by testicular tissue, and the serum concentrations of testosterone and oestradiol were then studied before and at 30 min after hCG injection. Intravenous injection of hCG induced a rapid increase in cAMP concentration and in the activity of cAMP-dependent protein kinase. The kinetics of this response indicated that cAMP and cAMP-dependent protein kinase mediate hCG effects on the human testis, presumably via effects on the Leydig cells. No stimulatory effect on steroid conversion in vitro or on the serum concentrations of testosterone and oestradiol were seen after 30 min.
十名不育男性接受了睾丸活检。在静脉注射人绒毛膜促性腺激素（1500 - 5000国际单位）之前以及注射后3分钟、10分钟、20分钟和30分钟获取的活检组织中，测定了环磷酸腺苷（cAMP）浓度和cAMP依赖性蛋白激酶活性。随后研究了hCG注射前及注射后30分钟睾丸组织对孕酮的体外转化情况，以及睾酮和雌二醇的血清浓度。静脉注射hCG导致cAMP浓度和cAMP依赖性蛋白激酶活性迅速增加。这种反应的动力学表明，cAMP和cAMP依赖性蛋白激酶大概是通过对睾丸间质细胞的作用来介导hCG对人睾丸的影响。30分钟后，未观察到对体外类固醇转化或睾酮和雌二醇血清浓度的刺激作用。
