De Valck V, Geerts A, Schellinck P, Wisse E
Laboratory for Cell Biology and Histology, Free University of Brussels (VUB), Belgium.
Histochemistry. 1988;89(4):357-63. doi: 10.1007/BF00500637.
In the present study we have localized neutral phosphatase, acid phosphatase, alkaline phosphatase and 5' nucleotidase in the sinusoidal cells of rat liver using enzyme cytochemistry at light and electron microscopical level. Neutral phosphatase was present in the endoplasmic reticulum and nuclear envelope of parenchymal cells and of sinusoidal endothelial, Kupffer and fat-storing cells. The intensity of the neutral phosphatase reaction was stronger in sinusoidal than in parenchymal cells. Sinusoidal cells were devoid of cytochemically demonstrable alkaline phosphatase. Abundant acid phosphatase was present in the many lysosomes of endothelial and Kupffer cells. Substantially less acid phosphatase-positive lysosomes were found in fat-storing cells. 5' nucleotidase was present on the cell membrane of fat-storing cells, on 90% of all Kupffer cells and on the microvilli of parenchymal cells. We have further shown that combined staining for 5' nucleotidase and for endogenous peroxidase, offers a histochemical tool to discriminate between the three main sinusoidal cell types in normal rat liver.
在本研究中，我们利用酶细胞化学技术，在光镜和电镜水平上对大鼠肝脏窦状隙细胞中的中性磷酸酶、酸性磷酸酶、碱性磷酸酶和5'-核苷酸酶进行了定位。中性磷酸酶存在于实质细胞、窦状隙内皮细胞、库普弗细胞和贮脂细胞的内质网和核膜中。窦状隙细胞中中性磷酸酶反应的强度比实质细胞中的更强。窦状隙细胞中没有细胞化学可显示的碱性磷酸酶。内皮细胞和库普弗细胞的许多溶酶体中存在大量酸性磷酸酶。贮脂细胞中酸性磷酸酶阳性溶酶体明显较少。5'-核苷酸酶存在于贮脂细胞的细胞膜上、所有库普弗细胞的90%以及实质细胞的微绒毛上。我们进一步表明，5'-核苷酸酶和内源性过氧化物酶的联合染色提供了一种组织化学工具，可用于区分正常大鼠肝脏中的三种主要窦状隙细胞类型。
