Renau-Piqueras J, Guerri C, Miragall F, Gómez-Perretta C, Báguena-Cervellera R
Virchows Arch B Cell Pathol Incl Mol Pathol. 1985;49(3):249-59. doi: 10.1007/BF02912102.
The activities of acid phosphatase, alkaline phosphatase, glucose-6-phosphatase, uridine diphosphatase, inosine diphosphatase, thiamine pyrophosphatase and 5'-nucleotidase have been investigated cytochemically in hepatocytes of the offspring of alcohol-fed rats, using cerium ions as a capturing agent and qualitative and quantitative electron microscopy. All these enzyme activities were decreased in the experimental animals compared with controls not exposed to ethanol. The pattern of deposition of the product of glucose-6-phosphatase activity in the cisternae of the endoplasmic reticulum was also different in the two groups. The phosphatases analyzed are functional markers of different cell components, and the results suggest that prenatal exposure of rats to ethanol causes functional alterations in the endoplasmic reticulum, Golgi apparatus, lysosomes and plasma membrane of hepatocytes.
利用铈离子作为捕获剂以及定性和定量电子显微镜技术，对酒精喂养大鼠后代的肝细胞进行了细胞化学研究，以检测酸性磷酸酶、碱性磷酸酶、葡萄糖-6-磷酸酶、尿苷二磷酸酶、肌苷二磷酸酶、硫胺素焦磷酸酶和5'-核苷酸酶的活性。与未接触乙醇的对照组相比，所有这些酶活性在实验动物中均降低。两组中内质网池内葡萄糖-6-磷酸酶活性产物的沉积模式也不同。所分析的磷酸酶是不同细胞成分的功能标志物，结果表明大鼠产前暴露于乙醇会导致肝细胞内质网、高尔基体、溶酶体和质膜的功能改变。
