Kim Joseph W, Vang Souriya, Luo John Zq, Newton William C, Luo Luguang
The Center of Stem Cell Biology, Department of Medicine, Roger Williams Hospital, Boston University, School of Medicine, Providence, RI 02908, USA.
The Center of Stem Cell Biology, Department of Medicine, Roger Williams Hospital, Boston University, School of Medicine, Providence, RI 02908, USA; Insure Health, Inc, 30 Quaker Lane Suite 35, Warwick, RI 02886, USA.
Mol Cell Endocrinol. 2017 Jul 15;450:32-42. doi: 10.1016/j.mce.2017.04.014. Epub 2017 Apr 17.
Stem cells are a new therapeutic modality that may support the viability and function of human organs and tissue. Our previous studies have revealed that human allogeneic bone marrow (BM) sustains pancreatic β cell function and survival. This paper examines whether BM creates a microenvironment that supports human pancreatic islets in vitro by evaluating 107 proteins in culture media from BM, islet, and islet/bone marrow (IB) with mass spectrometry. Proteins were considered up- or down-regulated if p-values < 0.05 and fold change was greater than 2 fold I VS. IB. In addition, proteins identified that were uniquely found in islets co-cultured with bone marrow, but not in islets or bone marrow. A 95% protein probability was used as a threshold. Twenty three proteins were upregulated, and sixteen proteins were downregulated. The function of each protein is listed based on the protein database, which include structural proteins (9 upregulated, 4 downregulated); anti-protease and anti-endopeptidase enzymes (8 upregulated); cation binding proteins (6 up-regulated). Six proteins were uniquely identified in islet co-cultured with bone marrow. Three are anti-proteases or anti-endopeptidases, and 1 is a structural protein. These findings suggest that BM, by changing culture media proteins, may be one of mechanisms to maintain human islet function and survival.
