单细胞RNA测序揭示了人类血液中新型树突状细胞、单核细胞和祖细胞。
Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors.
作者信息
Villani Alexandra-Chloé, Satija Rahul, Reynolds Gary, Sarkizova Siranush, Shekhar Karthik, Fletcher James, Griesbeck Morgane, Butler Andrew, Zheng Shiwei, Lazo Suzan, Jardine Laura, Dixon David, Stephenson Emily, Nilsson Emil, Grundberg Ida, McDonald David, Filby Andrew, Li Weibo, De Jager Philip L, Rozenblatt-Rosen Orit, Lane Andrew A, Haniffa Muzlifah, Regev Aviv, Hacohen Nir
机构信息
Broad Institute of MIT and Harvard, Cambridge, MA, USA.
Center for Cancer Research, Massachusetts General Hospital, Department of Medicine, Boston, MA, USA.
出版信息
Science. 2017 Apr 21;356(6335). doi: 10.1126/science.aah4573.
Dendritic cells (DCs) and monocytes play a central role in pathogen sensing, phagocytosis, and antigen presentation and consist of multiple specialized subtypes. However, their identities and interrelationships are not fully understood. Using unbiased single-cell RNA sequencing (RNA-seq) of ~2400 cells, we identified six human DCs and four monocyte subtypes in human blood. Our study reveals a new DC subset that shares properties with plasmacytoid DCs (pDCs) but potently activates T cells, thus redefining pDCs; a new subdivision within the CD1C subset of DCs; the relationship between blastic plasmacytoid DC neoplasia cells and healthy DCs; and circulating progenitor of conventional DCs (cDCs). Our revised taxonomy will enable more accurate functional and developmental analyses as well as immune monitoring in health and disease.
树突状细胞（DCs）和单核细胞在病原体感知、吞噬作用和抗原呈递中发挥核心作用，且由多种特殊亚型组成。然而，它们的特性和相互关系尚未完全明确。通过对约2400个细胞进行无偏差单细胞RNA测序（RNA-seq），我们在人血液中鉴定出六种人类DCs和四种单核细胞亚型。我们的研究揭示了一个新的DC亚群，它与浆细胞样DCs（pDCs）具有共同特性，但能有效激活T细胞，从而重新定义了pDCs；DCs的CD1C亚群内的一个新细分；母细胞性浆细胞样DC肿瘤细胞与健康DCs之间的关系；以及常规DCs（cDCs）的循环祖细胞。我们修订后的分类法将有助于进行更准确的功能和发育分析以及健康和疾病状态下的免疫监测。
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