Schleif R
Graduate Department of Biochemistry, Brandeis University, Waltham, MA 02254.
Science. 1988 Sep 2;241(4870):1182-7. doi: 10.1126/science.2842864.
Study of proteins that recognize specific DNA sequences has yielded much information, but the field is still in its infancy. Already two major structural motifs have been discovered, the helix-turn-helix and zinc finger, and numerous examples of DNA-binding proteins containing either of them are known. The restriction enzyme Eco RI uses yet a different motif. Additional motifs are likely to be found as well. There is a growing understanding of some of the physical chemistry involved in protein-DNA binding, but much remains to be learned before it becomes possible to engineer a protein that binds to a specific DNA sequence.
对识别特定DNA序列的蛋白质的研究已经产生了很多信息，但该领域仍处于起步阶段。已经发现了两种主要的结构基序，即螺旋-转角-螺旋和锌指，并且已知包含其中任何一种的DNA结合蛋白的大量实例。限制性内切酶Eco RI使用的是另一种不同的基序。很可能还会发现其他基序。人们对蛋白质与DNA结合所涉及的一些物理化学有了越来越多的了解，但在能够设计出一种与特定DNA序列结合的蛋白质之前，仍有许多有待学习的地方。
