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EcoRI限制酶-DNA复合物中的“交互式”识别

'Interactive' recognition in EcoRI restriction enzyme-DNA complex.

作者信息

Kim R, Modrich P, Kim S H

出版信息

Nucleic Acids Res. 1984 Oct 11;12(19):7285-92. doi: 10.1093/nar/12.19.7285.

DOI:10.1093/nar/12.19.7285
PMID:6093038
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC320161/
Abstract

A solution study of interaction between DNA and EcoRI restriction enzyme shows that there is a definite distortion of DNA in the specific recognition complexes but no measurable DNA distortion in the non-specific interaction.

摘要

一项关于DNA与EcoRI限制酶相互作用的溶液研究表明，在特异性识别复合物中DNA存在明确的扭曲，但在非特异性相互作用中没有可测量的DNA扭曲。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1003/320161/b35b8291b56d/nar00337-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1003/320161/ac04d547e1ac/nar00337-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1003/320161/b35b8291b56d/nar00337-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1003/320161/ac04d547e1ac/nar00337-0015-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1003/320161/b35b8291b56d/nar00337-0017-a.jpg

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本文引用的文献

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DNA supercoiling and its effects on DNA structure and function.DNA超螺旋及其对DNA结构和功能的影响。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:85-91. doi: 10.1101/sqb.1983.047.01.011.
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Kinked DNA in crystalline complex with EcoRI endonuclease.与EcoRI核酸内切酶形成晶体复合物的扭结DNA。
Nature. 1984;309(5966):327-31. doi: 10.1038/309327a0.
3
Thermodynamic parameters governing interaction of EcoRI endonuclease with specific and nonspecific DNA sequences.控制EcoRI核酸内切酶与特异性和非特异性DNA序列相互作用的热力学参数。
同时测定配体-DNA复合物中的螺旋解旋角和固有缔合常数：DNA与加利车霉素B之间的相互作用
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Site directed mutagenesis experiments suggest that Glu 111, Glu 144 and Arg 145 are essential for endonucleolytic activity of EcoRI.定点诱变实验表明，Glu 111、Glu 144和Arg 145对于EcoRI的核酸内切酶活性至关重要。
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alpha-Putrescinylthymine and the sensitivity of bacteriophage phi W-14 DNA to restriction endonucleases.α-腐胺基胸腺嘧啶与噬菌体φW-14 DNA对限制性核酸内切酶的敏感性
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Protein-induced unwinding of DNA: measurement by gel electrophoresis of complexes with DNA minicircles. Application to restriction endonuclease EcoRI, catabolite gene activator protein and lac repressor.蛋白质诱导的DNA解旋：通过与DNA小环复合物的凝胶电泳进行测量。应用于限制性内切酶EcoRI、分解代谢基因激活蛋白和乳糖阻遏物。
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Nucleotide excision repair in Escherichia coli.大肠杆菌中的核苷酸切除修复
Microbiol Rev. 1990 Mar;54(1):18-51. doi: 10.1128/mr.54.1.18-51.1990.
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Studies on sequence recognition by type II restriction and modification enzymes.II型限制与修饰酶的序列识别研究。
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Plasmids containing many tandem copies of a synthetic lactose operator.含有多个串联合成乳糖操纵子拷贝的质粒。
Gene. 1980 Feb;8(3):279-300. doi: 10.1016/0378-1119(80)90005-0.
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Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.氯霉素存在下大肠杆菌中Col E 1质粒复制的性质
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DNA-ethidium reaction kinetics: demonstration of direct ligand transfer between DNA binding sites.DNA-溴化乙锭反应动力学：DNA结合位点间直接配体转移的证明
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