Shanmugapriya Sakkaravarthy, Subramanian Perumal, Kanimozhi Sivamani
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Chidambaram, Tamil Nadu 608 002 India.
Indian J Clin Biochem. 2017 Jun;32(2):214-219. doi: 10.1007/s12291-016-0601-x. Epub 2016 Aug 6.
Endometrial carcinoma is the fourth most abundant cancer worldwide in women. Female Wistar rats were segregated into five groups: group I-control, group II-MNNG (N-methyl-N'-nitro-N-nitrosoguanidine-150 mg/kg) administered through intravaginal detention of cotton absorbent, group III-geraniol (GOH) only, group IV-GOH-pretreated (7 days before the start of MNNG administration); and group V-Co-administration of geraniol with MNNG. In this study, reverse transcriptase- PCR of K-ras, MAPK, PI3K, Wnt/β-catenin genes, TGF-β and expressions of PCNA, PTEN, progesterone receptor and E-cadherin by Western blotting were performed from endometrial cancer tissue and control tissues. The mRNA expressions of K-ras, MAPK, PI3K, Wnt/β-catenin and TGF-β were amplified in MNNG induced group. Oral administration of GOH (both pre and co-administration) reversed the mRNA expression towards normal. The reversibility is more predominant in pretreatment groups ( < 0.05). The expression of PCNA was upregulated and downregulation of PTEN, progesterone receptor and E-cadherin was noticed in MNNG induced rats. Pre and co-administration of GOH significantly reversed the expression pattern of proteins. GOH treatment is more effective in pretreatment groups ( < 0.05). These results provide powerful evidences that GOH could influence modulation of MAPK pathways and Wnt signalling pathways in the prevention of endometrial carcinoma in rats.
子宫内膜癌是全球女性中第四大常见癌症。将雌性Wistar大鼠分为五组：第一组为对照组，第二组通过阴道留置棉塞给予MNNG（N-甲基-N'-硝基-N-亚硝基胍，150 mg/kg），第三组仅给予香叶醇（GOH），第四组在开始给予MNNG前7天进行香叶醇预处理；第五组将香叶醇与MNNG联合给药。在本研究中，从子宫内膜癌组织和对照组织中进行了K-ras、MAPK、PI3K、Wnt/β-连环蛋白基因、TGF-β的逆转录聚合酶链反应以及通过蛋白质印迹法检测PCNA、PTEN、孕激素受体和E-钙黏蛋白的表达。在MNNG诱导组中，K-ras、MAPK、PI3K、Wnt/β-连环蛋白和TGF-β的mRNA表达被扩增。口服香叶醇（预处理和联合给药）均使mRNA表达恢复正常。在预处理组中这种可逆性更显著（P<0.05）。在MNNG诱导的大鼠中，PCNA表达上调，PTEN、孕激素受体和E-钙黏蛋白表达下调。香叶醇的预处理和联合给药显著逆转了蛋白质的表达模式。香叶醇治疗在预处理组中更有效（P<0.05）。这些结果提供了有力证据，表明香叶醇在预防大鼠子宫内膜癌中可影响MAPK通路和Wnt信号通路的调节。
