Levin G Ya, Sukhareva E G
Department of Gravitational Surgery and Hemodialysis, Volga Federal Medical Research Center, Ministry of Health of the Russian Federation, Nizhny Novgorod, Russia.
Bull Exp Biol Med. 2017 Apr;162(6):718-721. doi: 10.1007/s10517-017-3696-z. Epub 2017 Apr 21.
Coagulation and optical (based on chromogenic substrate) methods were employed to examine antithrombin activity of erythrocytes and erythrocyte-derived microvesicles isolated days 7, 14, 21, and 28 on erythrocyte storage. The erythrocyte-derived microvesicles decelerated fibrin clot formation from fibrinogen in the presence of exogenous thrombin both with and without heparin. Microvesicles reduced optical density of chromogenic substrate. These data suggest that erythrocyte-derived microvesicles display a prominent antithrombin activity, which significantly increases during erythrocyte storage.
采用凝血法和光学法（基于显色底物）检测在红细胞储存第7、14、21和28天分离出的红细胞及红细胞衍生微泡的抗凝血酶活性。在外源凝血酶存在的情况下，无论有无肝素，红细胞衍生微泡均能减缓纤维蛋白原形成纤维蛋白凝块的过程。微泡降低了显色底物的光密度。这些数据表明，红细胞衍生微泡具有显著的抗凝血酶活性，且在红细胞储存过程中该活性显著增加。
