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一种以金黄色葡萄球菌为标记物的抗原固相分析方法

[A method of solid-phase analysis of antigens using Staphylococcus aureus as the label].

作者信息

Khaustov V I, Shekoian L A, Drozdov S G, Korolev M B

出版信息

Vopr Virusol. 1988 Mar-Apr;33(2):196-201.

PMID:2842962
Abstract

A new method of solid-phase analysis of viral antigens in which St. aureus, strain Cowan 1, was used as a label is described. The result of analysis is read visually by bacterial agglutination. The degree of agglutination of the staphylococcal diagnosticum corresponds to the amount of the antigen bound with antibodies immobilized on the solid phase. Staining of Staphylococcus with methylene blue simplifies reading of the results. The method has been shown to be sufficiently simple, specific, sensitive, and may be used for diagnosis of different infections diseases.

摘要

描述了一种病毒抗原固相分析的新方法,其中使用 Cowan 1 株金黄色葡萄球菌作为标记物。通过细菌凝集以肉眼读取分析结果。葡萄球菌诊断菌的凝集程度与固定在固相上的抗体结合的抗原量相对应。用亚甲蓝对葡萄球菌进行染色可简化结果的读取。该方法已被证明足够简单、特异、灵敏,可用于诊断不同的感染性疾病。

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