Zhang Y H, Zhou D N, Wang C A, Hung T
Institute of Virology, Chinese Academy of Preventive Medicine, Beijing.
Acta Virol. 1989 Jan;33(1):32-8.
Staphylococcus aureus Cowan I rich in protein A when sensitized with guinea pig antiserum to adult diarrhea rotavirus (ADRV) at 1:16 gave a strong co-agglutination with ADRV-positive faecal samples as previously confirmed by electron microscopy (EM) and enzyme-linked immunosorbent assay (ELISA). The bacteria sensitized with normal guinea pig serum did not give any co-agglutination. Blocking tests using rabbit ADRV-specific antiserum for the treatment of twelve ADRV-positive samples abolished the reaction. All the fifty ELISA-confirmed ADRV-positive faecal samples gave positive co-agglutination, whereas all the forty-eight ELISA-negative faecal samples from healthy subjects gave negative results. The test has been proved to be rapid, simple, specific, and economic, useful for rapid diagnosis even in remote areas, so that the ADRV infection can definitely be differentiated from some of acute bacterial diarrheas.
金黄色葡萄球菌 Cowan I 富含A蛋白，当用豚鼠抗血清以1:16的比例与成人腹泻轮状病毒（ADRV）致敏时，与ADRV阳性粪便样本发生强烈的协同凝集反应，这一点先前已通过电子显微镜（EM）和酶联免疫吸附测定（ELISA）得到证实。用正常豚鼠血清致敏的细菌未出现任何协同凝集反应。使用兔ADRV特异性抗血清对12份ADRV阳性样本进行阻断试验，可消除反应。所有50份经ELISA确认的ADRV阳性粪便样本均产生阳性协同凝集反应，而来自健康受试者的48份ELISA阴性粪便样本均为阴性结果。该试验已被证明快速、简单、特异且经济，即使在偏远地区也有助于快速诊断，从而能明确将ADRV感染与某些急性细菌性腹泻区分开来。
