Cabirac G, Gilden D, Wellish M, Vafai A
Department of Neurology, University of Colorado School of Medicine, Denver 80262.
Virus Res. 1988 May;10(2-3):205-13. doi: 10.1016/0168-1702(88)90016-0.
BSC-1 cells infected with a vaccinia virus recombinant containing the coding sequences for varicella-zoster virus (VZV) glycoprotein I (gpI) were analyzed by indirect immunofluorescence and immunoprecipitation for the expression and processing of gpI. The processing of gpI in cells infected with recombinant virus was the same as that observed during VZV infection. Immunofluorescence revealed localization of gpI to the membranes of recombinant virus-infected cells.
用含有水痘-带状疱疹病毒(VZV)糖蛋白I(gpI)编码序列的痘苗病毒重组体感染的BSC-1细胞,通过间接免疫荧光和免疫沉淀法分析gpI的表达和加工过程。重组病毒感染细胞中gpI的加工过程与VZV感染期间观察到的相同。免疫荧光显示gpI定位于重组病毒感染细胞的膜上。