Sonotaki Seiichi, Takami Taku, Noguchi Keiichi, Odaka Masafumi, Yohda Masafumi, Murakami Yoshihiko
Department of Organic and Polymer Materials Chemistry, Tokyo University of Agriculture and Technology, 2-24-16 Naka-cho, Koganei-shi, Tokyo 184-8588, Japan.
Biomater Sci. 2017 May 30;5(6):1082-1089. doi: 10.1039/c7bm00207f.
We developed a hollow PEGylated encapsulin nanoparticle from Rhodococcus erythropolis N771. The hollow engineered encapsulin nanoparticles with His-Tag and Lys residues on the surface were constructed by means of genetic recombination. The Lys residues on the particle surface were successfully PEGylated with a PEG derivative, methoxy-PEG-SCM. Consequently, we demonstrated that the hollow PEGylated engineered encapsulin nanoparticle could successfully disassemble or reassemble even after PEGylation in the presence or absence of a protein denaturing agent. The nanoparticle obtained in the present study has the potential to incorporate hydrophilic compounds in the internal cavity of the particle by reversibly controllable disassembly and reassembly. The hollow PEGylated encapsulin nanoparticle can be used as a drug carrier for the delivery of hydrophilic biopolymers in future medical applications.
我们从红平红球菌N771开发了一种中空聚乙二醇化封装蛋白纳米颗粒。通过基因重组构建了表面带有His标签和赖氨酸残基的中空工程化封装蛋白纳米颗粒。颗粒表面的赖氨酸残基成功地用聚乙二醇衍生物甲氧基-聚乙二醇-琥珀酰亚胺基乙酸酯进行了聚乙二醇化。因此，我们证明了即使在有或没有蛋白质变性剂的情况下进行聚乙二醇化后，中空聚乙二醇化工程化封装蛋白纳米颗粒仍能成功地拆卸或重新组装。本研究中获得的纳米颗粒有可能通过可逆可控的拆卸和重新组装将亲水性化合物纳入颗粒的内腔。中空聚乙二醇化封装蛋白纳米颗粒在未来的医学应用中可用作递送亲水性生物聚合物的药物载体。
