碘化物对大鼠甲状腺（FRTL-5）细胞生长的抑制作用。

The inhibitory effect of iodide on growth of rat thyroid (FRTL-5) cells.

作者信息

Saji M, Isozaki O, Tsushima T, Arai M, Miyakawa M, Ohba Y, Tsuchiya Y, Sano T, Shizume K

机构信息

Department of Medicine, Institute of Clinical Endocrinology, Tokyo Women's Medical College, Japan.

出版信息

Acta Endocrinol (Copenh). 1988 Sep;119(1):145-51. doi: 10.1530/acta.0.1190145.

DOI:10.1530/acta.0.1190145

PMID:2842993

Abstract

The effect of iodide on growth of rat thyroid cells (FRTL-5) was studied. TSH-stimulated cell growth was inhibited by iodide in a concentration-dependent manner, and an effect of iodide was detected at 10(-6) mol/l. KClO4 or 1-methylimidazole-2-thiol blocked the effect of iodide, suggesting that iodide uptake and its organification are required to produce the inhibitory effect of iodide on cell growth. Iodide not only decreased TSH-stimulated cAMP production in FRTL-5 cells but also cell growth induced by cAMP. These observations suggest that iodide inhibits TSH-stimulated growth of the cells by attenuating cAMP production and also by acting on the step(s) distal to cAMP generation. The inhibitory effect of iodide was also seen in growth stimulated by insulin, insulin-like growth factor-I or 12-O-tetradecanoyl phorbol 13-acetate, suggesting multiple sites of action of iodide in the process of growth of FRTL-5 cells.

摘要

研究了碘化物对大鼠甲状腺细胞（FRTL-5）生长的影响。碘化物以浓度依赖的方式抑制促甲状腺激素（TSH）刺激的细胞生长，在10(-6)mol/L时可检测到碘化物的作用。高氯酸钾或1-甲基咪唑-2-硫醇可阻断碘化物的作用，提示碘化物摄取及其有机化是产生碘化物对细胞生长抑制作用所必需的。碘化物不仅降低FRTL-5细胞中TSH刺激的环磷酸腺苷（cAMP）生成，还降低由cAMP诱导的细胞生长。这些观察结果表明，碘化物通过减弱cAMP生成以及作用于cAMP生成的下游步骤来抑制TSH刺激的细胞生长。在胰岛素、胰岛素样生长因子-I或12-O-十四酰佛波醇-13-乙酸酯刺激的生长中也观察到碘化物的抑制作用，提示碘化物在FRTL-5细胞生长过程中有多个作用位点。