Cao Wei, Wu Xianjian, Jia Gang, Zhao Hua, Chen Xiaoling, Wu Caimei, Tang Jiayong, Wang Jing, Cai Jingyi, Liu Guangmang
a Institute of Animal Nutrition , Sichuan Agricultural University , Chengdu , China.
b Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education , Sichuan Agricultural University , Chengdu , China.
Arch Anim Nutr. 2017 Jun;71(3):175-191. doi: 10.1080/1745039X.2017.1314610.
This study aimed to determine the effects of dietary spermine supplementation on the inflammatory response and immune function of the thymus and spleen in piglets. Eighty suckling piglets were randomly assigned to receive adequate nutrients supplemented with spermine (0.4 mmol/kg body weight) or restricted nutrient intake supplemented with normal saline for 7 h or 3, 6 and 9 days in pairs. Regardless of treatment time, spermine supplementation decreased (p < 0.05, compared with the controls) the following: (1) tumour necrosis factor α (TNF-α), interleukin (IL)-1β, 2 and 6, and interferon (IFN)-γ levels in serum; (2) gene expression of cluster of differentiation 8 and integrin beta-2 in the thymus and spleen and the lymphocyte function-associated antigen 1 in the thymus; (3) mRNA levels of TNF-α, IL 1β, 2, 6, and 12, IFN-γ and inducible nitric oxide synthase in the thymus and spleen, as well as IL-8 in the spleen; and (4) eukaryotic IF4E-binding protein 1, Janus kinase 2, signal transducer and activator of transcription 3, and nuclear factor-kappa B P65 gene transcriptions in the thymus and spleen. By contrast, spermine supplementation increased (p < 0.05) the following: (1) immunoglobulin M, IL-10, and transforming growth factor β1 gene expression, as well as (2) relative mRNA levels of mammalian target of rapamycin (mTOR) and ribosomal protein S6 kinase 1 in the thymus and spleen. These effects were also observed upon prolonged spermine administration (p < 0.05). In summary, dietary spermine supplementation can alleviate inflammatory response, enhance the immune function and regulate the gene expression of signalling molecules related to inflammation.
本研究旨在确定日粮中添加精胺对仔猪胸腺和脾脏炎症反应及免疫功能的影响。80头哺乳仔猪被随机分为两组，一组接受补充精胺（0.4 mmol/kg体重）的充足营养，另一组接受补充生理盐水的限饲，每组分别处理7小时或3、6、9天。无论处理时间如何，添加精胺均降低了（与对照组相比，p < 0.05）以下指标：（1）血清中肿瘤坏死因子α（TNF-α）、白细胞介素（IL）-1β、2和6以及干扰素（IFN）-γ水平；（2）胸腺和脾脏中分化簇8和整合素β-2以及胸腺中淋巴细胞功能相关抗原1的基因表达；（3）胸腺和脾脏中TNF-α、IL 1β、2、6和12、IFN-γ以及诱导型一氧化氮合酶的mRNA水平，以及脾脏中IL-8的mRNA水平；（4）胸腺和脾脏中真核IF4E结合蛋白1、Janus激酶2、信号转导和转录激活因子3以及核因子κB P65基因的转录。相比之下，添加精胺增加了（p < 0.05）以下指标：（1）免疫球蛋白M、IL-10和转化生长因子β1的基因表达，以及（2）胸腺和脾脏中雷帕霉素靶蛋白（mTOR）和核糖体蛋白S6激酶1的相对mRNA水平。长期给予精胺也观察到了这些效果（p < 0.05）。总之，日粮中添加精胺可减轻炎症反应，增强免疫功能并调节与炎症相关信号分子의基因表达。
