Liu Guangmang, Zheng Jie, Cao Wei, Wu Xianjian, Jia Gang, Zhao Hua, Chen Xiaoling, Wu Caimei, Wang Jing
Institute of Animal Nutrition, Sichuan Agricultural University Chengdu 611130 Sichuan China
Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education Chengdu 611130 Sichuan China.
RSC Adv. 2019 Apr 9;9(20):11054-11062. doi: 10.1039/c8ra05421e.
This study investigated the effects of spermine supplementation and its extended duration on amino acid transporters, immune status, barrier function, and apoptosis in the liver. Eighty piglets were randomly assigned to a group receiving either a diet supplemented with spermine (0.4 mmol kg of body weight) or a restricted nutrient intake supplemented with saline in pairs for 7 h, 3 days, 6 days, and 9 days. Regardless of treatment time, spermine increased the levels of amino acid transporters, immunoglobulin M, antimicrobial peptides, cellular immune components, anti-inflammatory cytokines, mammalian target of rapamycin, ribosomal protein S6 kinase 1, signal transducer and activator of transcription 2 and 3, Janus kinase 2, zonula occludens 1 and 2, occluding, claudin-1, claudin-2, claudin-16 and Bcl-2 mRNA levels, whereas it decreased the levels of pro-inflammatory cytokines, inducible nitric oxide synthase, nuclear factor-kappa B P65, eukaryotic IF4E-binding protein 1, myosin light chain kinase, Bax, and caspase-3 mRNA in the liver ( < 0.05). These effects were also found in cases of prolonged spermine intake ( < 0.05). Spermine can decrease pro-inflammatory cytokines and caspase-3 levels. In conclusion, spermine may promote barrier function and improve amino acid transport, and can increase immune status and inhibit apoptosis in the liver.
本研究调查了补充精胺及其延长给药时间对肝脏中氨基酸转运体、免疫状态、屏障功能和细胞凋亡的影响。80只仔猪被随机分为两组，一组接受补充精胺（0.4 mmol/kg体重）的日粮，另一组接受补充生理盐水的限制营养摄入，每组配对饲养7小时、3天、6天和9天。无论治疗时间如何，精胺均可提高氨基酸转运体、免疫球蛋白M、抗菌肽、细胞免疫成分、抗炎细胞因子、雷帕霉素靶蛋白、核糖体蛋白S6激酶1、信号转导和转录激活因子2和3、Janus激酶2、紧密连接蛋白1和2、闭合蛋白、claudin-1、claudin-2、claudin-16和Bcl-2的mRNA水平，而降低肝脏中促炎细胞因子、诱导型一氧化氮合酶、核因子-κB P65、真核IF4E结合蛋白1、肌球蛋白轻链激酶、Bax和caspase-3的mRNA水平（P<0.05）。在延长精胺摄入的情况下也发现了这些作用（P<0.05）。精胺可降低促炎细胞因子和caspase-3水平。总之，精胺可能促进屏障功能，改善氨基酸转运，并可提高免疫状态，抑制肝脏细胞凋亡。
