Thorne-Tjomsland G, Clermont Y, Hermo L
Department of Anatomy, McGill University, Montreal, Canada.
Anat Rec. 1988 Jun;221(2):591-8. doi: 10.1002/ar.1092210205.
The cytochemical distribution of two different acid phosphatases, nicotinamide adenine dinucleotide phosphatase (NADPase) and cytidine monophosphatase (CMPase), was analyzed within the Golgi apparatus, acrosomic system, and chromatoid body of early spermatids. Within the saccules composing the Golgi cortex, the phosphatases were localized in specific and nonoverlapping compartments: the cis-tubular network, or cis-element, and the first underlying saccule were unreactive for both enzymes, the following four to five saccules were reactive for NADPase, and the last three saccules were reactive for CMPase. Vesicles of various sizes and short membranous tubules, present near the edges of saccules and in the medulla of the Golgi apparatus, were NADPase- and/or CMPase-positive. The acrosomic system showed a weak but significant NADPase reactivity and a strong CMPase reactivity. The vesicles associated with the chromatoid body were reactive for NADPase and/or CMPase. The combined observations indicate that the two cytochemically distinct compartments of the Golgi cortex, the NADPase- and CMPase-positive saccules, independently contribute to the formation of the acrosomic system and the vesicular component of the chromatoid body.
分析了早期精子细胞的高尔基体、顶体系统和类核体中两种不同酸性磷酸酶，即烟酰胺腺嘌呤二核苷酸磷酸酶（NADPase）和胞苷单磷酸酶（CMPase）的细胞化学分布。在构成高尔基体皮质的扁平囊泡中，磷酸酶定位于特定且不重叠的区室：顺式管状网络或顺式元件，以及其下方的第一个扁平囊泡对两种酶均无反应，接下来的四到五个扁平囊泡对NADPase有反应，最后三个扁平囊泡对CMPase有反应。在扁平囊泡边缘附近和高尔基体髓质中存在的各种大小的囊泡和短膜性小管呈NADPase和/或CMPase阳性。顶体系统显示出较弱但显著的NADPase反应性和较强的CMPase反应性。与类核体相关的囊泡对NADPase和/或CMPase有反应。综合观察表明，高尔基体皮质在细胞化学上不同的两个区室，即NADPase和CMPase阳性的扁平囊泡，分别独立地参与顶体系统和类核体囊泡成分的形成。
