Hennigar R A, Sens D A, Othersen H B, Garvin A J
Department of Pathology, Medical University of South Carolina, Charleston 29425.
Arch Pathol Lab Med. 1988 Sep;112(9):908-13.
Two alpha-fucose-binding lectins, Ulex europaeus agglutinin I (UEA I) and Lotus tetragonolobus agglutinin, were employed to compare and contrast the distribution of fucosubstance in normal human kidneys and a variety of renal tumors. The study employed a total of 31 kidneys surgically removed for the presence of a variety of tumors, including 11 unilateral Wilms' tumors, two cases of bilateral Wilms' tumors, 13 renal cell carcinomas, two congenital mesoblastic nephromas, one renal oncocytoma, one neuroblastoma metastatic to the kidney, and one clear cell sarcoma of the kidney. The results show that UEA I-reactive fucosubstance is detected in vascular endothelium of all kidneys and tumors, except bilateral Wilms' tumors. The presence of UEA I-reactive alpha-fucose in the vasculature of unilateral but not bilateral Wilms' tumors defines a unique histochemical distinction between the two groups of tumors. Conceivably, this property might be exploited as a screening procedure for the more aggressive bilateral neoplasms. Other findings detail histochemical differences between UEA I and L tetragonolobus agglutinin, as evidenced by the ability of one lectin to stain a particular cell type that is not reactive with the other lectin.
使用两种α-岩藻糖结合凝集素，即欧洲荆豆凝集素I（UEA I）和四角豆凝集素，来比较和对比正常人类肾脏及多种肾肿瘤中岩藻糖物质的分布。该研究共使用了31个因各种肿瘤而手术切除的肾脏，包括11例单侧肾母细胞瘤、2例双侧肾母细胞瘤、13例肾细胞癌、2例先天性中胚叶肾瘤、1例肾嗜酸细胞瘤、1例转移至肾脏的神经母细胞瘤以及1例肾透明细胞肉瘤。结果显示，除双侧肾母细胞瘤外，在所有肾脏和肿瘤的血管内皮中均检测到UEA I反应性岩藻糖物质。单侧而非双侧肾母细胞瘤血管中存在UEA I反应性α-岩藻糖，这定义了两组肿瘤之间独特的组织化学差异。可以想象，这一特性可能被用作一种筛查方法，用于筛查更具侵袭性的双侧肿瘤。其他发现详细阐述了UEA I和四角豆凝集素之间的组织化学差异，一种凝集素能够染色特定细胞类型而另一种凝集素无反应即证明了这一点。
