一种用于快速一步克隆全长类病毒cDNA的改进方法。
An improved procedure for the rapid one-step-cloning of full-length viroid cDNA.
作者信息
Puchta H, Sänger H L
机构信息
Max-Planck-Institut für Biochemie, Abteilung Viroidforschung, Martinsried bei München, Federal Republic of Germany.
出版信息
Arch Virol. 1988;101(1-2):137-40. doi: 10.1007/BF01314659.
PMID:2843147
Abstract
The efficiency of viroid cloning can be increased by three to four orders of magnitude when the synthesis of viroid cDNA is primed in such a way that it carries identical sticky ends on both termini and when the multi-ion transformation is applied.
摘要
当以在两端携带相同粘性末端的方式引发类病毒cDNA的合成并应用多离子转化时，类病毒克隆的效率可以提高三到四个数量级。
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