Cress D E, Kiefer M C, Owens R A
Nucleic Acids Res. 1983 Oct 11;11(19):6821-35. doi: 10.1093/nar/11.19.6821.
Contiguous restriction fragments from two cloned partial-length potato spindle tuber viroid (PSTV) cDNAs were used to construct recombinant DNAs containing full-length monomeric and dimeric PSTV cDNA. When five different PSTV cDNA plasmids and RNA isolated from E. coli cells harboring these plasmids were tested for infectivity on tomato, plasmid DNAs containing PSTV cDNA dimers were infectious. RNA transcripts containing the sequence of PSTV from these plasmids were also infectious. The sequences of the viroid progeny and the cloned DNA were identical. In vitro mutagenesis of infectious PSTV cDNAs will allow systematic investigation of the role of specific sequences in viroid replication and pathogenesis.
来自两个克隆的部分长度马铃薯纺锤块茎类病毒（PSTV）cDNA的相邻限制片段被用于构建包含全长单体和二聚体PSTV cDNA的重组DNA。当测试五种不同的PSTV cDNA质粒以及从携带这些质粒的大肠杆菌细胞中分离出的RNA对番茄的感染性时，含有PSTV cDNA二聚体的质粒DNA具有感染性。来自这些质粒的包含PSTV序列的RNA转录本也具有感染性。类病毒子代和克隆DNA的序列是相同的。对具有感染性的PSTV cDNA进行体外诱变将有助于系统研究特定序列在类病毒复制和发病机制中的作用。
