细胞色素aa3的一种新的高电位氧化还原转变。
A new high potential redox transition for cytochrome aa3.
作者信息
Hendler R W, Sidhu G S
机构信息
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, Bethesda, Maryland 20892.
出版信息
Biophys J. 1988 Jul;54(1):121-33. doi: 10.1016/S0006-3495(88)82937-0.
Using cyano-complexes of iron, tungsten, and molybdenum and a platinum working electrode, we have been able to attain and hold voltages in the range of 400 to 900 mV (vs. standard hydrogen electrode) in an aqueous medium. With this system we have obtained additional information in support of an earlier conclusion that cytochrome a3 has a high Em transition (i.e. greater than 460 mV) in addition to its Em in the 180-200 mV range (Hendler, R. W., K. V. S. Reddy, R. I. Shrager, and W. S. Caughey. 1986. Biophys. J. 49:717-729; Reddy, K. V. S., and R. W. Hendler. 1986. Biophys. J. 49:693-703). The proposed new transition has an Em near 770 mV and an n value greater than 1. The reduced form of the high-potential species of cytochrome a3 does not bind CO, in contrast to the reduced form of the low-potential species which does. A possible reaction scheme for cytochrome aa3 which incorporates the new information is presented.
使用铁、钨和钼的氰基配合物以及铂工作电极，我们能够在水介质中达到并保持400至900毫伏（相对于标准氢电极）范围内的电压。利用该系统，我们获得了更多信息，以支持早期的一个结论，即细胞色素a3除了在180 - 200毫伏范围内有其标准电极电位（Em）外，还具有高Em跃迁（即大于460毫伏）（亨德勒，R. W.，K. V. S. 雷迪，R. I. 施拉格，和W. S. 考希。1986年。《生物物理杂志》49:717 - 729；雷迪，K. V. S.，和R. W. 亨德勒。1986年。《生物物理杂志》49:693 - 703）。所提出的新跃迁的Em接近770毫伏且n值大于1。与能结合一氧化碳的低电位形式的还原态相反，细胞色素a3的高电位形式的还原态不结合一氧化碳。本文给出了一个纳入新信息的细胞色素aa3的可能反应方案。
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