一种来自糙海参的高度规则岩藻糖基硫酸软骨素：结构与凝血效应。
A highly regular fucosylated chondroitin sulfate from the sea cucumber Massinium magnum: Structure and effects on coagulation.
机构信息
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 119991, Russia.
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, Moscow 119991, Russia.
出版信息
Carbohydr Polym. 2017 Jul 1;167:20-26. doi: 10.1016/j.carbpol.2017.02.101. Epub 2017 Feb 27.
A fucosylated chondroitin sulfate MM was isolated from the sea cucumber Massinium magnum. Structure of this polysaccharide was determined using chemical and NMR spectroscopic methods. The backbone of MM was shown to consist mainly of chondroitin sulfate E units with a small portion (about 10%) of chondroitin sulfate A fragments. Practically one type of branches Fuc3S4S attached to O-3 of GlcA residues was found in the polysaccharide molecules. The main repeating units of MM are →4)-[α-l-Fuc3S4S-(1→3)]-β-d-GlcA-(1→3)-β-d-GalNAc4S6S-(1→, whereas the minor repeating units are →4)-[α-l-Fuc3S4S-(1→3)]-β-d-GlcA-(1→3)-β-d-GalNAc4S-(1→. Anticoagulant activity of MM determined in APTT and TT tests was shown to be lower than that of heparin, but higher than that of enoxaparin. In the experiments with purified proteins MM effectively potentiated inhibition of thrombin and factor Xa by ATIII. Besides, MM did not induce platelets aggregation in platelets rich plasma.
从海参 Massinium magnum 中分离得到一种岩藻糖基硫酸软骨素 MM。该多糖的结构通过化学和 NMR 光谱方法确定。结果表明，MM 的骨架主要由硫酸软骨素 E 单元组成，其中含有一小部分（约 10%）的硫酸软骨素 A 片段。在多糖分子中发现了几乎只有一种类型的 Fuc3S4S 分支连接到 GlcA 残基的 O-3 上。MM 的主要重复单元为 →4)-[α-l-Fuc3S4S-(1→3)]-β-d-GlcA-(1→3)-β-d-GalNAc4S6S-(1→，而次要重复单元为 →4)-[α-l-Fuc3S4S-(1→3)]-β-d-GlcA-(1→3)-β-d-GalNAc4S-(1→。在 APTT 和 TT 试验中测定的 MM 的抗凝活性低于肝素，但高于依诺肝素。在与纯化蛋白的实验中，MM 可有效增强 ATIII 对凝血酶和因子 Xa 的抑制作用。此外，MM 不会在富含血小板的血浆中诱导血小板聚集。
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