通过协同调节氧化还原电位和氧气摄取提高球形红细菌中辅酶Q的产量
Synergic regulation of redox potential and oxygen uptake to enhance production of coenzyme Q in Rhodobacter sphaeroides.
作者信息
Zhu Yongqiang, Ye Lidan, Chen Zhaofeng, Hu Weijiang, Shi Yanghui, Chen Jianbo, Wang Chenfei, Li Yong, Li Weifeng, Yu Hongwei
机构信息
Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, PR China.
Group of Bioengineering, ZheJiang NHU Company Limited, Shaoxing 312521, PR China.
出版信息
Enzyme Microb Technol. 2017 Jun;101:36-43. doi: 10.1016/j.enzmictec.2017.03.005. Epub 2017 Mar 15.
The physiological role of Coenzyme Q (CoQ) as an electron carrier suggests its association with redox potential. Overexpression of glyceraldehyde-3-phosphate dehydrogenase type I (gapA-1) in Rhodobacter sphaeroides elevated the NADH/NAD ratio and meanwhile enhanced the CoQ content by 58%, but at the sacrifice of biomass. On the other hand, Vitreoscilla hemoglobin was heterologously expressed to enhance the oxygen uptake ability of the cells, leading to 127% improvement of biomass. Subsequent coexpression of gapA-1 and vgb resulted in a CoQ titer of 83.24mg/L, representing 71% improvement as compared to the control strain RspMCS. When gapA-1 and vgb genes were co-expressed in a previously created strain RspMQd [1], 163.5mg/L of CoQ was produced. Finally, 600mg/L of CoQ production was achieved in fed-batch fermentation. These results demonstrated the synergic effect of redox potential regulation and oxygen uptake improvement on enhancing CoQ production in R. sphaeroides.
辅酶Q（CoQ）作为电子载体的生理作用表明其与氧化还原电位有关。球形红细菌中I型甘油醛-3-磷酸脱氢酶（gapA-1）的过表达提高了NADH/NAD比率，同时使CoQ含量提高了58%，但以生物量为代价。另一方面，透明颤菌血红蛋白的异源表达提高了细胞的氧气摄取能力，使生物量提高了127%。随后gapA-1和vgb的共表达导致CoQ滴度达到83.24mg/L，与对照菌株RspMCS相比提高了71%。当gapA-1和vgb基因在先前构建的菌株RspMQd [1]中共表达时，产生了163.5mg/L的CoQ。最后，在补料分批发酵中实现了600mg/L的CoQ产量。这些结果证明了氧化还原电位调节和氧气摄取改善对提高球形红细菌中CoQ产量的协同作用。
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