Liu Kuan-Ju
Department of Food Science, National Penghu University of Science and Technology, Makung City 88046, Penghu, Taiwan, ROC.
Enzyme Microb Technol. 2017 Jun;101:51-56. doi: 10.1016/j.enzmictec.2017.03.004. Epub 2017 Mar 18.
Enzymatic syntheses of oligosaccharide fatty acid esters are important owing to their wide range of industrial applications in the food, cosmetic, and pharmaceutical industries. Transesterification of isomaltotriose and palmitic acid vinyl ester, catalyzed by the metalloprotease thermolysin, was performed in organic solvents. The process parameters (reaction time and temperature) were optimized to achieve the highest yield of isomaltotriose palmitate (IP). The water content of the reaction system played a key role in the acylation of isomaltotriose. Dimethyl sulfoxide was thought to be the most suitable reaction medium by taking the degree of substitution of the modified isomaltotriose into account. The optimum reaction time, temperature, water content, and enzyme concentration were 24h, 45°C, 40%, and 0.05%, respectively, under which the product yield was as high as 89.7%. The enzyme operational stability study showed that thermolysin retained 51.5% of its initial activity for the synthesis of IP (even after repeated use for 72h). Moreover, test results showed that the emulsifying capacity and emulsion stability of IP are 107.5mL oil/g ester and 16.3%, respectively.
由于低聚糖脂肪酸酯在食品、化妆品和制药行业具有广泛的工业应用，因此其酶促合成具有重要意义。在有机溶剂中，由金属蛋白酶嗜热菌蛋白酶催化异麦芽三糖与棕榈酸乙烯酯进行酯交换反应。对工艺参数（反应时间和温度）进行了优化，以实现异麦芽三糖棕榈酸酯（IP）的最高产率。反应体系的含水量在异麦芽三糖的酰化反应中起关键作用。考虑到改性异麦芽三糖的取代度，二甲基亚砜被认为是最合适的反应介质。最佳反应时间、温度、含水量和酶浓度分别为24小时、45℃、40%和0.05%，在此条件下产物产率高达89.7%。酶的操作稳定性研究表明，嗜热菌蛋白酶在IP合成中保留了其初始活性的51.5%（即使重复使用72小时后）。此外，测试结果表明，IP的乳化能力和乳液稳定性分别为107.5mL油/克酯和16.3%。
