Salgarella Alice Rita, Cafarelli Andrea, Ricotti Leonardo, Capineri Lorenzo, Dario Paolo, Menciassi Arianna
The BioRobotics Institute, Scuola Superiore Sant'Anna, Pontedera (Pisa), Italy.
The BioRobotics Institute, Scuola Superiore Sant'Anna, Pontedera (Pisa), Italy.
Ultrasound Med Biol. 2017 Jul;43(7):1452-1465. doi: 10.1016/j.ultrasmedbio.2017.03.003. Epub 2017 Apr 19.
Described here is an in vitro systematic investigation of the effects on C2C12 myoblasts of exposure to finely controlled and repeatable low-intensity pulsed ultrasound of different frequencies (500 kHz, 1 MHz, 3 MHz and 5 MHz) and different intensities (250, 500 and 1000 mW/cm). An in-house stimulation system and an ultrasound-transparent cell culture well minimized reflections and attenuations, allowing precise control of ultrasound delivery. Results indicated that a 3 MHz stimulation at 1 W/cm intensity maximized cell proliferation in comparison with the other exposure conditions and untreated controls. In contrast, cell differentiation and the consequent formation of multinucleated myotubes were maximized by 1 MHz stimulation at 500 mW/cm intensity. The highly controlled exposure conditions employed allowed precise correlation of the ultrasound delivery to the bio-effects produced, thus overcoming the inconsistency of some results available in the literature and contributing to the potential of ultrasound treatment for muscle therapy and regeneration.
本文描述了一项体外系统研究，该研究针对不同频率（500kHz、1MHz、3MHz和5MHz）和不同强度（250、500和1000mW/cm²）的精细控制且可重复的低强度脉冲超声对C2C12成肌细胞的影响。一个内部刺激系统和一个超声透明的细胞培养孔可将反射和衰减降至最低，从而实现对超声传递的精确控制。结果表明，与其他暴露条件和未处理的对照组相比，1W/cm²强度的3MHz刺激可使细胞增殖最大化。相反，500mW/cm²强度的1MHz刺激可使细胞分化以及随后多核肌管的形成最大化。所采用的高度可控的暴露条件使得超声传递与所产生的生物效应能够精确关联，从而克服了文献中一些结果的不一致性，并为超声治疗肌肉疾病和再生的潜力做出了贡献。
