Kartashov Mikhail Yu, Glushkova Ludmila I, Mikryukova Tamara P, Korabelnikov Igor V, Egorova Yulia I, Tupota Natalia L, Protopopova Elena V, Konovalova Svetlana N, Ternovoi Vladimir A, Loktev Valery B
State Research Center for Virology and Biotechnology "Vector", Department of Molecular Virology for Flaviviruses and Viral Hepatitis, Koltsovo, Novosibirsk Region, Russia; Novosibirsk State University, Novosibirsk, Russia; Tomsk State University, Tomsk, Russia.
Department of Rospotrebnadzor in the Komi Republic, Syktyvkar, Russia.
Ticks Tick Borne Dis. 2017 Jun;8(4):588-592. doi: 10.1016/j.ttbdis.2017.04.001. Epub 2017 Apr 6.
The number of tick-borne infections in the northern European regions of Russia has increased considerably in the last years. In the present study, 676 unfed adult Ixodes persulcatus ticks were collected in the Komi Republic from 2011 to 2013 to study tick-borne rickettsioses. Rickettsia spp. DNA was detected by PCR in 51 (7.6%) ticks. The nucleotide sequence analysis of gltA fragments (765bp) from 51 ticks indicated that 60.8% and 39.2% of the ticks were infected with Rickettsia helvetica and Candidatus R. tarasevichiae, respectively. The gltA fragments showed 100% identity with those of Candidatus R. tarasevichiae previously discovered in Siberia and China, whereas R. helvetica showed 99.9% sequence identity with European isolates. The ompB had 8 nucleotide substitutions, 6 of which resulted in amino acid substitutions. In the sca9 gene, 3 nucleotide substitutions were detected, and only one resulted in amino acid substitution. The smpA, ompW, and β-lactamase genes of R. helvetica also showed a high level of sequence identity.
在过去几年中，俄罗斯北欧地区蜱传播感染的数量大幅增加。在本研究中，2011年至2013年期间在科米共和国采集了676只未进食的成年全沟硬蜱，以研究蜱传播的立克次体病。通过PCR在51只（7.6%）蜱中检测到立克次体属DNA。对51只蜱的gltA片段（765bp）进行核苷酸序列分析表明，分别有60.8%和39.2%的蜱感染了瑞士立克次体和类塔拉塞维奇立克次体。gltA片段与先前在西伯利亚和中国发现的类塔拉塞维奇立克次体的片段具有100%的同一性，而瑞士立克次体与欧洲分离株的序列同一性为99.9%。ompB有8个核苷酸替换，其中6个导致氨基酸替换。在sca9基因中，检测到3个核苷酸替换，只有1个导致氨基酸替换。瑞士立克次体的smpA、ompW和β-内酰胺酶基因也显示出高度的序列同一性。
