Inokuma Hisashi, Ohashi Misato, Tanabe Shigeyuki, Miyahara Kazuro
Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
J Vet Med Sci. 2007 Jun;69(6):661-4. doi: 10.1292/jvms.69.661.
DNA from 111 ticks collected by flagging in Tokachi district, Eastern Hokkaido, Japan were examined for infection with Rickettsia and Ehrlichia, by PCR and sequencing methodology. For Rickettsia, analysis of the partial sequence of the citrate synthase gene was successfully performed on 11 DNA samples from I. persulcatus, and 7 of them showed 99.8% identical with Rickettsia helvetica while the other 4 showed 99.8% identical with ;Candidatus Rickettsia tarasevichiae'. For Ehrlichia, a partial sequence of the 16S rRNA gene detected from I. persulcatus was 100% identical with that from Ehrlichia muris, and another DNA sample from I. ovatus showed 99.8% identical with Ehrlichia species detected from I. ovatus. The results suggest that the pathogens detected here might be distributed in this area.
通过PCR和测序方法，对在日本北海道东部十胜地区通过拖旗法采集的111只蜱虫的DNA进行了立克次氏体和埃立克体感染检测。对于立克次氏体，成功对来自全沟硬蜱的11个DNA样本进行了柠檬酸合酶基因部分序列分析，其中7个样本与瑞士立克次氏体的序列一致性为99.8%，另外4个样本与“塔拉舍维奇埃立克体（暂定种）”的序列一致性为99.8%。对于埃立克体，从全沟硬蜱中检测到的16S rRNA基因部分序列与鼠埃立克体的序列100%相同，另一个来自卵形硬蜱的DNA样本与从卵形硬蜱中检测到的埃立克体物种的序列一致性为99.8%。结果表明，此处检测到的病原体可能在该地区分布。
