Albuquerque Priscilla B S, Soares Paulo A G, Aragão-Neto Adelmo C, Albuquerque Giwellington S, Silva Luís C N, Lima-Ribeiro Maria H M, Silva Neto Jacinto C, Coelho Luana C B B, Correia Maria T S, Teixeira José A C, Carneiro-da-Cunha Maria G
Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Campus Universitário, s/n, Cidade Universitária, CEP: 50670-901, Recife, PE, Brazil; Departamento de Bioquímica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, s/n, Cidade Universitária, CEP: 50670-420, Recife, PE, Brazil.
Instituto de Bioquímica Médica Leopoldo de Meis and Hospital Universitário Clementino Fraga Filho, Programa de Glicobiologia, Universidade Federal do Rio de Janeiro, CEP: 21941-913, Rio de Janeiro, RJ, Brazil.
Int J Biol Macromol. 2017 Sep;102:749-757. doi: 10.1016/j.ijbiomac.2017.04.064. Epub 2017 Apr 19.
Galactomannan films from Cassia grandis seeds, associated or not with Cramoll 1,4, were used on topical wounds of rats for the evaluation of the healing process during 14days. All of the films were evaluated by cytotoxic assay, FTIR and lectin hemagglutinating activity (HA). Forty-five male rats were submitted to aseptic dermal wounds (Ø=0.8cm) and divided in groups (n=15): control, test 1, and test 2, treated respectively with saline, galactomannan film and film with immobilized Cramoll 1,4. Macroscopic evaluations were performed by clinical observations and area measurements, and microscopic analysis by histological criteria. Epithelial cell proliferation and differentiation was immunohistochemically assessed using CK14 and PCNA. The presence of CO peaks in the FTIR spectrum confirmed the immobilization of Cramoll 1,4 in the film, while the residual HA confirmed the stability of the lectin after immobilization with 90.94% of the initial HA. The films presented non-cytotoxicity and cell viability exceeding 80%. All of the animals presented re-epithelization around 10days, furthermore test 2 group showed a diffuse response at the stromal tissue and the basal layer associated with wounds completely closed with 11days of experiment. The results suggest a promising use of the films as topical wound curatives.
来自决明子种子的半乳甘露聚糖薄膜，无论是否与Cramoll 1,4结合，都被用于大鼠的局部伤口，以评估14天内的愈合过程。所有薄膜均通过细胞毒性测定、傅里叶变换红外光谱（FTIR）和凝集素血凝活性（HA）进行评估。45只雄性大鼠接受无菌皮肤伤口（直径=0.8厘米），并分为几组（n=15）：对照组、试验1组和试验2组，分别用生理盐水、半乳甘露聚糖薄膜和固定有Cramoll 1,4的薄膜进行治疗。通过临床观察和面积测量进行宏观评估，通过组织学标准进行微观分析。使用细胞角蛋白14（CK14）和增殖细胞核抗原（PCNA）免疫组织化学评估上皮细胞增殖和分化。FTIR光谱中CO峰的存在证实了Cramoll 1,4在薄膜中的固定，而残留的HA证实了凝集素在固定后的稳定性，初始HA的90.94%得以保留。这些薄膜表现出无细胞毒性且细胞活力超过80%。所有动物在大约10天时出现重新上皮化，此外，试验2组在基质组织和基底层表现出弥漫性反应，在实验11天时伤口完全闭合。结果表明这些薄膜有望作为局部伤口治疗剂使用。
