生长中的、汇合的和转化的3T3细胞中纤溶酶原激活物的细胞内分布差异。
Differences in intracellular distribution of plasminogen activator in growing, confluent, and transformed 3T3 cells.
作者信息
Jaken S, Black P H
出版信息
Proc Natl Acad Sci U S A. 1979 Jan;76(1):246-50. doi: 10.1073/pnas.76.1.246.
Abstract
The intracellular distribution of plasminogen activator in growing, confluent, and transformed 3T3 cells was investigated by using sucrose density gradient centrifugation. In confluent cells, which do not release plasminogen activator extracellularly, most of the activity was found in a heavy membrane fraction. In growing and transformed 3T3 cells, which do release plasminogen activator extracellularly in large amounts, most of the activity was found in lighter, plasma membrane-enriched fractions. A correlation between its presence in the light membrane fractions and the ability of the cells to release the plasminogen activator extracellularly is made.
摘要
通过蔗糖密度梯度离心法研究了纤溶酶原激活剂在生长、汇合及转化的3T3细胞中的细胞内分布。在不向细胞外释放纤溶酶原激活剂的汇合细胞中，大部分活性存在于重膜组分中。在大量向细胞外释放纤溶酶原激活剂的生长及转化的3T3细胞中，大部分活性存在于较轻的、富含质膜的组分中。由此建立了其在轻膜组分中的存在与细胞向细胞外释放纤溶酶原激活剂能力之间的关联。
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