利用 Nannochloropsis oculata 和 Isochrysis galbana 微藻油进行酶法酸解生产新型人乳脂肪替代品。
Production of new human milk fat substitutes by enzymatic acidolysis of microalgae oils from Nannochloropsis oculata and Isochrysis galbana.
机构信息
College of Life Science, Fujian Normal University, Fuzhou 350117, China.
Department of Engineering, Aarhus University, Gustav Wieds Vej 10, 8000 Aarhus C, Denmark.
出版信息
Bioresour Technol. 2017 Aug;238:129-138. doi: 10.1016/j.biortech.2017.04.041. Epub 2017 Apr 12.
Human milk fat substitutes (HMFs) with four kinds of n-3 fatty acid for infant formula were firstly synthesized using triacylglycerols (TAGs) from Nannochloropsis oculata rich in PA at the sn-2 position and free fatty acids (FFAs) from Isochrysis galbana rich in n-3 polyunsaturated fatty acids (n-3 PUFAs-ALA/SDA/DHA) via solvent-free acidolysis with Novozym 435, Lipozyme 435, TL-IM and RM-IM as biocatalysts. The results show that the resulting HMFs contain total n-3 PUFA of 13.92-17.12% and PA of 59.38-68.13% at the sn-2 position under the optimal conditions (mole ratio FFAs/TAG 3:1, 60°C (Novozym 435 and Lipozyme TL-IM) and 50°C (Lipozyme 435 and RM-IM), lipase loading 10%, reaction time 24h). Moreover, among the tested enzymes, Lipozyme 435, TL-IM, and RM-IM display the fatty acid selectivity towards SDA, LA and ALA, and OA, respectively. Overall, the examined lipases are promising biocatalysts for producing high-value microalgal HMFs in a cost-effective manner.
首次使用富含多不饱和脂肪酸（PUFA）的 Nannochloropsis oculata 的三酰基甘油(TAGs)和富含 n-3 多不饱和脂肪酸(n-3 PUFA-ALA/SDA/DHA)的 Isochrysis galbana 的游离脂肪酸(FFAs)作为酰基供体，通过 Novozym 435、Lipozyme 435、TL-IM 和 RM-IM 作为生物催化剂，在无溶剂条件下酸解合成了 4 种 n-3 脂肪酸的人乳脂肪替代品(HMFs)。结果表明，在最佳条件下（FFAs/TAG 摩尔比为 3:1，60°C（Novozym 435 和 Lipozyme TL-IM）和 50°C（Lipozyme 435 和 RM-IM），脂肪酶用量为 10%，反应时间 24h），所得 HMFs 在 sn-2 位含有总 n-3PUFA13.92%-17.12%和 PA59.38%-68.13%。此外，在所测试的酶中，Lipozyme 435、TL-IM 和 RM-IM 对 SDA、LA 和 ALA 以及 OA 分别表现出脂肪酸选择性。总之，所研究的脂肪酶是一种很有前途的生物催化剂，可用于以经济有效的方式生产高附加值的微藻 HMFs。
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