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[在铜绿假单胞菌BS205菌株染色体中发现的Tn3611和Tn3613转座子的特征]

[Characteristics of Tn3611 and Tn3613 transposons discovered in the chromosome of the BS205 strain of Pseudomonas aeruginosa].

作者信息

Erova T E, Anisimova L A, Kochetkov V V, Boronin A M

出版信息

Genetika. 1988 May;24(5):781-90.

PMID:2843420
Abstract

The transposons Tn3611 (HgR, 9.0 kb) and Tn3613 (SmRCmRKmRSuRHgR, 24.0 kb) were discovered in the chromosome of Pseudomonas aeruginosa BS205. The physical and genetic maps of these transposons were constructed using restriction endonucleases EcoRI, BamHI, SalI, HindIII. Genes of the mer operon, tnpA and tnpR were localized in the Tn3611 transposon, the genes DHPS-II, aad, tnpA, tnpR being situated in the map of the Tn3613 transposon. It was established that the Tn3611 belongs to the class of Tn3-like transposons and the Tn3613 is a complex transposon flanked by inverted repeats 2.2 kb in size, the transposon comprising both Tn3611 and a migrating sequence.

摘要

转座子Tn3611(汞抗性,9.0 kb)和Tn3613(链霉素-庆大霉素-卡那霉素-磺胺-汞抗性,24.0 kb)是在铜绿假单胞菌BS205染色体中发现的。使用限制性内切酶EcoRI、BamHI、SalI、HindIII构建了这些转座子的物理图谱和遗传图谱。汞操纵子基因、tnpA和tnpR定位于Tn3611转座子中,二氢蝶酸合酶-II基因、aad、tnpA、tnpR位于Tn3613转座子图谱中。已确定Tn3611属于Tn3样转座子类别,Tn3613是一个复杂转座子,两侧是大小为2.2 kb的反向重复序列,该转座子包含Tn3611和一个迁移序列。

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