Azorín-Vega E, Rojas-Calderón E, Martínez-Ventura B, Ramos-Bernal J, Serrano-Espinoza L, Jiménez-Mancilla N, Ordaz-Rosado D, Ferro-Flores G
Gerencia de Aplicaciones Nucleares en la Salud, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México 52750, Mexico.
Gerencia de Ciencias Ambientales, Instituto Nacional de Investigaciones Nucleares, Ocoyoacac, Estado de México 52750, Mexico.
Appl Radiat Isot. 2018 Aug;138:73-77. doi: 10.1016/j.apradiso.2017.04.016. Epub 2017 Apr 12.
The aim of this research was to evaluate the cell cycle redistribution and activation of early and late apoptotic pathways in lymphoma cells after treatment with Lu-anti-CD20. Experimental and computer models were used to calculate the radiation absorbed dose to cancer cell nuclei. The computer model (Monte Carlo, PENELOPE) consisted of twenty spheres representing cells with an inner sphere (cell nucleus) embedded in culture media. Radiation emissions of the radiopharmaceutical located in cell membranes and in culture media were considered for nuclei dose calculations. Flow cytometric analyses demonstrated that doses as low as 4.8Gy are enough to induce cell cycle arrest and activate late apoptotic pathways.
本研究的目的是评估用镥标记的抗CD20治疗后淋巴瘤细胞的细胞周期重新分布以及早期和晚期凋亡途径的激活情况。使用实验模型和计算机模型来计算癌细胞核的辐射吸收剂量。计算机模型（蒙特卡洛，PENELOPE）由二十个球体组成，代表细胞，其中内部球体（细胞核）嵌入培养基中。在计算细胞核剂量时考虑了位于细胞膜和培养基中的放射性药物的辐射发射。流式细胞术分析表明，低至4.8Gy的剂量足以诱导细胞周期停滞并激活晚期凋亡途径。
