Ivanišević Jasmina, Kotur-Stevuljević Jelena, Stefanović Aleksandra, Miljković Milica, Jelić-Ivanović Zorana, Pejović Biljana, Peco-Antić Amira
Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia.
Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia.
Chem Biol Interact. 2017 Jun 25;272:47-52. doi: 10.1016/j.cbi.2017.04.014. Epub 2017 Apr 20.
Acute kidney injury (AKI) is defined as a decrease in glomerular filtration rate with an increase in serum creatinine (sCr). Perinatal asphyxia (PNA) may be etiological factor for AKI with oxidative stress also implicated. Paraoxonase 1 (PON1) activity has been reported to be decreased in renal disease. The aim of our study was to evaluate paraoxonase 1 (PON1) activity and oxidative stress during the first hours and first days of life and to determine if these parameters could discriminate neonates having AKI from those who do not.
Serum samples at different time points after birth were obtained from 64 preterm newborns with PNA (45 defined as having AKI, 19 as non-AKI). Clinical markers, sCr, total oxidant status (TOS), total antioxidant status (TAS) and PON1 activity were measured.
The AKI group had more newborns with hypoxic ischemic encephalopathy, significantly higher serum creatinine (sCr) at 3 and 7d, total antioxidant status (TAS) at 7d; decreased PON1 at 4h, 6h and 7d than the non-AKI group. Within the AKI group, significant positive correlations were found between PON1 activity at 2h and TAS at 2h, PON1 activity at 4h and base deficit (BD); whereas negative correlations between PON1 activity at 2h and ΔsCr (at 24h and at 3d), PON1 activity at 7d and ΔsCr (at 24h and 3d). Oxidative stress status parameters indicated excellent discriminative potential at 4h, 6h and 7d.
AKI neonates were characterised by a marked decrease in PON1 activity. PON1 activity may be an important factor for discrimination of newborns having AKI from those that do not.
急性肾损伤（AKI）定义为肾小球滤过率降低伴血清肌酐（sCr）升高。围产期窒息（PNA）可能是AKI的病因，氧化应激也与之相关。据报道，对氧磷酶1（PON1）活性在肾脏疾病中降低。我们研究的目的是评估出生后头几个小时和头几天的对氧磷酶1（PON1）活性和氧化应激，并确定这些参数是否能区分患有AKI的新生儿和未患AKI的新生儿。
从64例患有PNA的早产新生儿（45例定义为患有AKI，19例为非AKI）中获取出生后不同时间点的血清样本。测量临床指标、sCr、总氧化状态（TOS）、总抗氧化状态（TAS）和PON1活性。
与非AKI组相比，AKI组有更多新生儿患有缺氧缺血性脑病，在第3天和第7天血清肌酐（sCr）显著更高，在第7天总抗氧化状态（TAS）更高；在4小时、6小时和7天时PON1降低。在AKI组中，发现2小时时的PON1活性与2小时时的TAS之间、4小时时的PON1活性与碱缺失（BD）之间存在显著正相关；而2小时时的PON1活性与ΔsCr（24小时和3天时）、7天时的PON1活性与ΔsCr（24小时和3天时）之间存在负相关。氧化应激状态参数在4小时、6小时和7天时显示出良好的鉴别潜力。
AKI新生儿的特征是PON1活性显著降低。PON1活性可能是区分患有AKI的新生儿和未患AKI的新生儿的一个重要因素。
