Rodrigues Ana I, Reis Rui L, van Blitterswijk Clemens A, Leonor Isabel B, Habibović Pamela
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco GMR, Portugal.
ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal.
Biomater Res. 2017 Apr 19;21:6. doi: 10.1186/s40824-017-0092-8. eCollection 2017.
Bioinorganics have been explored as additives to ceramic bone graft substitutes with the aim to improve their performance in repair and regeneration of large bone defects. Silicon (Si), an essential trace element involved in the processes related to bone formation and remodeling, was shown not only to enhance osteoblasts proliferation but also to stimulate the differentiation of mesenchymal stem cells (MSCs) and preosteoblasts into the osteogenic lineage. In this study, the added value of Si to calcium phosphate (CaP) coatings was evaluated.
Tissue culture plastic well plates were coated with a thin CaP layer to which traces amounts of Si were added, either by adsorption or by incorporation through coprecipitation. The physicochemical and structural properties of the coatings were characterized and the dissolution behavior was evaluated. The adsorption/incorporation of Si was successfully achieved and incorporated ions were released from the CaP coatings. Human MSCs were cultured on the coatings to examine the effects of Si on cell proliferation and osteogenic differentiation. For the statistical analysis, a one-way ANOVA with Bonferroni post-hoc test was performed.
The results showed that human MSCs (hMSCs) responded to the presence of Si in the CaP coatings, in a dose-dependent manner. An increase in the expression of markers of osteogenic differentiation by human MSCs was observed as a result of the increase in Si concentration.
The incorporation/adsorption of Si into CaP coatings was successfully achieved and hMSCs responded with an increase in osteogenic genes expression with the increase of Si concentration. Furthermore, hMSCs cultured on CaP-I coatings expressed higher levels of ALP and OP, indicating that this may be the preferred method of incorporation of bioinorganics into CaPs.
生物无机化合物已被研究作为陶瓷骨移植替代物的添加剂，旨在提高其在修复和再生大骨缺损方面的性能。硅（Si）是一种参与骨形成和重塑相关过程的必需微量元素，不仅能促进成骨细胞增殖，还能刺激间充质干细胞（MSC）和前成骨细胞向成骨谱系分化。在本研究中，评估了硅对磷酸钙（CaP）涂层的附加值。
在组织培养塑料孔板上涂覆一层薄的CaP层，通过吸附或共沉淀掺入痕量的硅。对涂层的物理化学和结构性质进行表征，并评估其溶解行为。成功实现了硅的吸附/掺入，并且掺入的离子从CaP涂层中释放出来。将人MSC培养在涂层上，以研究硅对细胞增殖和成骨分化的影响。进行统计学分析时，采用单因素方差分析和Bonferroni事后检验。
结果表明，人MSC（hMSC）对CaP涂层中硅的存在呈剂量依赖性反应。随着硅浓度的增加，观察到hMSC成骨分化标志物的表达增加。
成功实现了硅掺入/吸附到CaP涂层中，并且hMSC随着硅浓度的增加，成骨基因表达增加。此外，在CaP-I涂层上培养的hMSC表达更高水平的碱性磷酸酶（ALP）和骨钙素（OP），表明这可能是将生物无机化合物掺入CaP的首选方法。
