Aizawa "Next-generation Bioceramics" Project, Kanagawa Academy of Science and Technology (KAST), 3-2-1 Sakado, Kanagawa, Kawasaki, 2130012, Japan.
J Mater Sci Mater Med. 2012 Dec;23(12):2923-32. doi: 10.1007/s10856-012-4744-x. Epub 2012 Aug 14.
The influence of silicon-substituted hydroxyapatite (Si-HAp) on osteogenic differentiation was assessed by biological analysis. Si-HAp was prepared by ultrasonic spray pyrolysis (USSP) technique using various amounts of Si (0, 0.8, and 1.6 mass%). Chemical analysis revealed that Si was incorporated into the hydroxyapatite (HAp) lattice with no other crystalline phase and which caused the change of crystal structure. Biological analyses showed that the Si contents affected the cell proliferation and morphology, suggesting that there is an optimal Si content for cell culture. As for differentiation, alkaline phosphatase activity and osteocalcin production of Si-HAp were higher than those of HAp. Gene expression profiles also revealed that substitution of Si (0.8 mass%) up-regulated the expression levels of osteocalcin and especially Runx2, a master gene for osteoblast development. These results suggest that incorporating Si into the HAp lattice may enhance the bioactivity, particularly during early osteoblast development.
采用超声喷雾热解法（USSP）技术，通过添加不同量的硅（0、0.8 和 1.6 质量%）来制备硅取代羟基磷灰石（Si-HAp），并对其成骨分化的影响进行生物学分析。化学分析表明，硅掺入到羟基磷灰石（HAp）晶格中，没有其他晶相，从而导致晶体结构发生变化。生物学分析表明，硅含量影响细胞增殖和形态，表明细胞培养存在最佳硅含量。至于分化，Si-HAp 的碱性磷酸酶活性和骨钙素生成均高于 HAp。基因表达谱也表明，硅（0.8 质量%）取代可上调骨钙素的表达水平，特别是成骨细胞发育的主基因 Runx2。这些结果表明，将硅掺入 HAp 晶格中可能会增强生物活性，特别是在早期成骨细胞发育过程中。
