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一种新型海洋细菌sp. JS-C42,具有糖化植物生物质以辅助生产纤维素乙醇的能力。

A novel marine bacterium sp. JS-C42 with the ability to saccharifying the plant biomasses for the aid in cellulosic ethanol production.

作者信息

Santhi Velayudhan Satheeja, Gupta Ashutosh, Saranya Somasundaram, Jebakumar Solomon Robinson David

机构信息

Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai 625 021, Tamil Nadu, India.

出版信息

Biotechnol Rep (Amst). 2014 May 17;1-2:8-14. doi: 10.1016/j.btre.2014.05.002. eCollection 2014 Jun.

Abstract

The ever growing demands for food products such as starch and sugar produces; there is a need to find the sources for saccharification for cellulosic bioethanol production. This study provides the first evidence of the lignocellulolytic and saccharifying ability of a marine bacterium namely sp. JS-C42, a Gram positive actinobacterium with the cocci cells embedded on mycelia isolated from the Arabian Sea, India. It exhibited highest filter paper unit effect, endoglucanase, exoglucanase, cellobiohydrolase, β-glucosidase, xylanase and ligninase effect. The hydrolytic potential of the enzymes displayed the efficient saccharification capability of steam pretreated biomass. It was also found to degrade the paddy, sorghum, and into simple reducing sugars by its efficient lignocellulose enzyme complex with limited consumption of sugars. Production of ethanol was also achieved with the . Overall, it offers a great potential for the cellulosic ethanol production in an economically reliable and eco-friendly point-of-care.

摘要

对淀粉和糖等食品的需求不断增长;因此需要寻找用于纤维素生物乙醇生产糖化的来源。本研究首次证明了一种海洋细菌即JS-C42菌的木质纤维素分解和糖化能力,该菌是一种革兰氏阳性放线菌,其球菌细胞嵌入从印度阿拉伯海分离的菌丝体中。它表现出最高的滤纸酶活力、内切葡聚糖酶、外切葡聚糖酶、纤维二糖水解酶、β-葡萄糖苷酶、木聚糖酶和木质素酶活力。这些酶的水解潜力显示出蒸汽预处理生物质的高效糖化能力。还发现它能通过其高效的木质纤维素酶复合物将水稻、高粱降解为简单的还原糖,且糖的消耗有限。利用该菌也实现了乙醇的生产。总体而言,它在经济可靠且环保的即时护理方面为纤维素乙醇生产提供了巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c8d2/5381695/c4cea768465b/gr1.jpg

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