在L细胞悬浮培养中高诱导白藜芦醇的处理策略。

Treatment strategies for high resveratrol induction in L. cell suspension culture.

作者信息

Vuong Thu V, Franco Chris, Zhang Wei

机构信息

Department of Medical Biotechnology, School of Medicine, Flinders University, Adelaide 5042, Australia.

Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada.

出版信息

Biotechnol Rep (Amst). 2014 May 19;1-2:15-21. doi: 10.1016/j.btre.2014.04.002. eCollection 2014 Jun.

Bioprocesses capable of producing large scales of resveratrol at nutraceutical grade are in demand. This study herein investigated treatment strategies to induce the production of resveratrol in L. cell suspension cultures. Among seven investigated elicitors, jasmonic acid (JA), salicylic acid, β-glucan (GLU), and chitosan enhanced the production of intracellular resveratrol manyfold. The combined treatment of JA and GLU increased extracellular resveratrol production by up to tenfold. The application of Amberlite XAD-7 resin for removal and artificial storage of secreted resveratrol further increased resveratrol production by up to four orders of magnitude. The level of resveratrol produced in response to the combined treatment with 200 g/L XAD-7, 10 μM JA and 1 mg/mL GLU was approximately 2400 mg/L, allowing the production of resveratrol at an industrial scale. The high yield of resveratrol is due to the involvement of a number of mechanisms working in concert.

需要能够以营养保健品级大规模生产白藜芦醇的生物工艺。本研究在此调查了诱导白藜芦醇在L.细胞悬浮培养物中产生的处理策略。在七种研究的诱导子中，茉莉酸（JA）、水杨酸、β-葡聚糖（GLU）和壳聚糖使细胞内白藜芦醇的产量提高了许多倍。JA和GLU的联合处理使细胞外白藜芦醇产量提高了多达十倍。应用Amberlite XAD-7树脂去除和人工储存分泌的白藜芦醇，进一步使白藜芦醇产量提高了多达四个数量级。响应于200 g/L XAD-7、10 μM JA和1 mg/mL GLU的联合处理产生的白藜芦醇水平约为2400 mg/L，从而能够实现工业规模的白藜芦醇生产。白藜芦醇的高产率归因于多种协同作用机制的参与。