Weinmaster G A, Hunter T
Molecular Biology and Virology Laboratory, Salk Institute, San Diego, California 92138.
J Virol. 1988 Oct;62(10):3849-54. doi: 10.1128/JVI.62.10.3849-3854.1988.
Changing Glu-1025 to Asp in Fujinami sarcoma virus P130gag-fps made the protein temperature sensitive for transformation and protein-tyrosine kinase activity. Another mutant, Phe-1073 P130gag-fps, lacking the major autophosphorylation site, has an extended latent period for transformation (G. A. Weinmaster, M. J. Zoller, M. Smith, E. Hinze, and T. Pawson, Cell 37:559-568, 1984). By introducing the Asp-1025 lesion into Phe-1073 P130gag-fps, we showed that this mutant protein is required for the maintenance of the transformed phenotype of Phe-1073 P130gag-fps-expressing cells.
将 Fujinami 肉瘤病毒 P130gag-fps 中的 Glu-1025 替换为 Asp，会使该蛋白对转化和蛋白酪氨酸激酶活性产生温度敏感性。另一个突变体，即缺乏主要自磷酸化位点的 Phe-1073 P130gag-fps，其转化的潜伏期延长（G. A. Weinmaster、M. J. Zoller、M. Smith、E. Hinze 和 T. Pawson，《细胞》37:559 - 568，1984 年）。通过将 Asp-1025 损伤引入 Phe-1073 P130gag-fps，我们发现这种突变蛋白对于维持表达 Phe-1073 P130gag-fps 的细胞的转化表型是必需的。
