微管相关蛋白对秋水仙碱与微管蛋白结合的竞争性抑制作用。
Competitive inhibition of colchicine binding to tubulin by microtubule-associated proteins.
作者信息
Nunez J, Fellous A, Francon J, Lennon A M
出版信息
Proc Natl Acad Sci U S A. 1979 Jan;76(1):86-90. doi: 10.1073/pnas.76.1.86.
Abstract
Microtubule-associated proteins (MAPs) promote tubulin polymerization, whereas colchicine inhibits this process. In this paper, MAPs have been shown to inhibit colchicine binding to tubulin in a competitive manner. Attempts were made to identify which of the MAPs fraction(s) was responsible; both tau protein (a thermostable molecule with a molecular weight of approximately 70,000) and a high molecular weight fraction (HMW) were able to compete with colchicine. In contrast, Mg2+, which also induces microtubule assembly in vitro, had no effect on colchicine binding to tubulin.
摘要
微管相关蛋白（MAPs）促进微管蛋白聚合，而秋水仙碱抑制这一过程。在本文中，已表明MAPs以竞争性方式抑制秋水仙碱与微管蛋白的结合。人们试图确定哪个MAPs组分起作用；tau蛋白（一种分子量约为70,000的热稳定分子）和高分子量组分（HMW）都能够与秋水仙碱竞争。相比之下，同样能在体外诱导微管组装的Mg2+对秋水仙碱与微管蛋白的结合没有影响。
相似文献
1
Competitive inhibition of colchicine binding to tubulin by microtubule-associated proteins.微管相关蛋白对秋水仙碱与微管蛋白结合的竞争性抑制作用。
Proc Natl Acad Sci U S A. 1979 Jan;76(1):86-90. doi: 10.1073/pnas.76.1.86.
2
Molecules in mammalian brain that interact with the colchicine site on tubulin.哺乳动物大脑中与微管蛋白上秋水仙碱结合位点相互作用的分子。
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1184-8. doi: 10.1073/pnas.76.3.1184.
3
Endogenous inhibitor of colchicine-tubulin binding in rat brain.大鼠脑中秋水仙碱-微管蛋白结合的内源性抑制剂。
Science. 1979 Aug 10;205(4406):593-5. doi: 10.1126/science.451622.
4
Binding of dihydroxynaphthyl aryl ketones to tubulin colchicine site inhibits microtubule assembly.二羟基萘基芳基酮与微管蛋白秋水仙碱位点的结合会抑制微管组装。
Biochem Biophys Res Commun. 2015 Oct 23;466(3):418-25. doi: 10.1016/j.bbrc.2015.09.041. Epub 2015 Sep 11.
5
Polymerising ability of C6 glial cell microtubule protein decays much faster than its colchicine-binding activity.C6神经胶质细胞微管蛋白的聚合能力比其秋水仙碱结合活性衰退得快得多。
Nature. 1977 Sep 29;269(5627):435-6. doi: 10.1038/269435a0.
6
In vitro assembly of pure tubulin into microtubules in the absence of microtubule-associated proteins and glycerol.在没有微管相关蛋白和甘油的情况下，将纯微管蛋白在体外组装成微管。
Proc Natl Acad Sci U S A. 1977 May;74(5):1860-4. doi: 10.1073/pnas.74.5.1860.
7
A quantitative description of microtubule formation in the presence of tubulin-colchicine.
Eur J Biochem. 1983 May 2;132(2):369-73. doi: 10.1111/j.1432-1033.1983.tb07372.x.
8
Kinetic and steady-state analysis of microtubules in the presence of colchicine.秋水仙碱存在下微管的动力学和稳态分析
Biochemistry. 1981 Apr 14;20(8):2316-24. doi: 10.1021/bi00511a038.
9
Polymerization of the tubulin-colchicine complex: relation to microtubule assembly.微管蛋白 - 秋水仙碱复合物的聚合作用：与微管组装的关系
Biochemistry. 1983 Mar 29;22(7):1556-66. doi: 10.1021/bi00276a006.
10
Colchicine-binding protein of the liver. Its characterization and relation to microtubules.肝脏的秋水仙碱结合蛋白。其特性及与微管的关系。
J Cell Biol. 1975 Sep;66(3):609-20. doi: 10.1083/jcb.66.3.609.
引用本文的文献
1
Coagulopathy in COVID-19 and anticoagulation clinical trials.新型冠状病毒肺炎合并凝血病与抗凝临床试验
Best Pract Res Clin Haematol. 2022 Sep;35(3):101377. doi: 10.1016/j.beha.2022.101377. Epub 2022 Aug 23.
2
Exploring the potential role of tungsten carbide cobalt (WC-Co) nanoparticle internalization in observed toxicity toward lung epithelial cells in vitro.探讨碳化钨钴（WC-Co）纳米颗粒内化在体外观察到的对肺上皮细胞毒性中的潜在作用。
Toxicol Appl Pharmacol. 2014 Jul 1;278(1):1-8. doi: 10.1016/j.taap.2014.04.008. Epub 2014 Apr 16.
3
Anti-microtubule activity of tubeimoside I and its colchicine binding site of tubulin.土贝母苷甲的抗微管活性及其与微管蛋白的秋水仙碱结合位点
Cancer Chemother Pharmacol. 2008 Sep;62(4):559-68. doi: 10.1007/s00280-007-0635-0. Epub 2007 Nov 21.
4
Structural and functional alterations in microtubule protein from Chinese hamster ovary cell mutants.中国仓鼠卵巢细胞突变体中微管蛋白的结构和功能改变
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5638-42. doi: 10.1073/pnas.78.9.5638.
5
Effect of tau on the vinblastine-induced aggregation of tubulin.微管相关蛋白tau对长春花碱诱导的微管蛋白聚集的影响。
J Cell Biol. 1981 Jun;89(3):680-3. doi: 10.1083/jcb.89.3.680.
6
Regulatory aspects of the colchicine interactions with tubulin.
Mol Cell Biochem. 1987 Jan;73(1):29-36. doi: 10.1007/BF00229373.
7
Microtubule-associated proteins-dependent colchicine stability of acetylated cold-labile brain microtubules from the Atlantic cod, Gadus morhua.大西洋鳕鱼（Gadus morhua）脑中乙酰化冷不稳定微管的微管相关蛋白依赖性秋水仙碱稳定性
J Cell Biol. 1991 Apr;113(2):331-8. doi: 10.1083/jcb.113.2.331.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The mechanism of action of colchicine. Binding of colchincine-3H to cellular protein.秋水仙碱的作用机制。秋水仙碱-3H与细胞蛋白的结合。
J Cell Biol. 1967 Aug;34(2):525-33. doi: 10.1083/jcb.34.2.525.
3
The colchicine-binding protein of mammalian brain and its relation to microtubules.哺乳动物脑内的秋水仙碱结合蛋白及其与微管的关系。
Biochemistry. 1968 Dec;7(12):4466-79. doi: 10.1021/bi00852a043.
4
Properties of colchicine binding protein from chick embryo brain. Interactions with vinca alkaloids and podophyllotoxin.鸡胚脑秋水仙碱结合蛋白的特性。与长春花生物碱和鬼臼毒素的相互作用。
Biochemistry. 1970 Dec 8;9(25):4999-5007. doi: 10.1021/bi00827a026.
5
Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.通过在SDS-聚丙烯酰胺凝胶上进行电泳来测定分子量。
Methods Enzymol. 1972;26:3-27. doi: 10.1016/s0076-6879(72)26003-7.
6
Microtuble assembly: some possible regulatory mechanisms.微管组装：一些可能的调控机制。
J Supramol Struct. 1974;2(2-4):429-50. doi: 10.1002/jss.400020230.
7
Microtubule assembly in the absence of added nucleotides.在未添加核苷酸的情况下微管装配。
Proc Natl Acad Sci U S A. 1973 Mar;70(3):765-8. doi: 10.1073/pnas.70.3.765.
8
Turbidimetric studies of the in vitro assembly and disassembly of porcine neurotubules.猪神经微管体外组装与拆卸的比浊法研究。
J Mol Biol. 1974 Nov 15;89(4):737-55. doi: 10.1016/0022-2836(74)90048-5.
9
Tubulin requires an accessory protein for self assembly in microtubules.微管蛋白在微管中自我组装需要一种辅助蛋白。
Nature. 1975 Oct 2;257(5525):418-21. doi: 10.1038/257418a0.
10
Microtubules and brain development.微管与大脑发育。
FEBS Lett. 1975 Sep 1;57(1):5-8. doi: 10.1016/0014-5793(75)80139-6.
Zotero 插件