Barochovsky O, Docherty M, Bradford H F
Department of Biochemistry, Imperial College of Science and Technology, London, U.K.
Neurosci Lett. 1988 Aug 1;90(3):320-7. doi: 10.1016/0304-3940(88)90209-1.
When differentiated cells of the cholinergic NS-20Y type were incubated with an antiserum to choline acetyltransferase (ChAT), in the presence of complement, immunolysis occurred as demonstrated by release of 20% of total lactate dehydrogenase and 70% of total ChAT. No significant immunolytic effects were observed when either undifferentiated (dividing or non-dividing) or partially differentiated cells were incubated under identical conditions. When the Triton X-114 phase separation technique was employed using membranes from differentiated cells, a small but significant proportion of choline acetyltransferase was recovered in the detergent-rich phase. These results suggest that a membrane-bound form of ChAT is a surface marker of the NS-20Y cell line.
当胆碱能NS - 20Y型分化细胞与抗胆碱乙酰转移酶（ChAT）抗血清在补体存在的情况下孵育时，会发生免疫溶解，这可通过总乳酸脱氢酶的20%和总ChAT的70%释放来证明。当未分化（分裂或不分裂）或部分分化的细胞在相同条件下孵育时，未观察到明显的免疫溶解作用。当使用分化细胞的膜采用Triton X - 114相分离技术时，在富含去污剂的相中回收了一小部分但比例显著的胆碱乙酰转移酶。这些结果表明，ChAT的膜结合形式是NS - 20Y细胞系的表面标志物。
