Suppr超能文献

通过高效液相色谱法(HPLC)和通用核糖体PCR(URP-PCR)分析对新培育的高产虫草素菌株进行表征

Characterization of Newly Bred Strains for Higher Production of Cordycepin through HPLC and URP-PCR Analysis.

作者信息

Lee Hyun-Hee, Kang Naru, Park Inmyoung, Park Jungwook, Kim Inyoung, Kim Jieun, Kim Namgyu, Lee Jae-Yun, Seo Young-Su

机构信息

Department of Microbiology, Pusan National University, Busan 46241, Republic of Korea.

CMG Biofarm, Busan 46958, Republic of Korea.

出版信息

J Microbiol Biotechnol. 2017 Jul 28;27(7):1223-1232. doi: 10.4014/jmb.1701.01043.

Abstract

, a member of Ascomycota, a mushroom referred to as caterpillar Dongchung-ha-cho, is commercially valuable because of its high content of bioactive substances, including cordycepin, and its potential for artificial cultivation. Cordycepin (3'-deoxyadenosine) is highly associated with the pharmacological effects of . is heterothallic in that two mating-type loci, idiomorph and , exist discretely in two different spores. In this study, nine strains were mated with each other to prepare newly bred strains that produced a larger amount of cordycepin than the parent strains. Nine strains of were identified by comparing the internal transcribed spacer sequence, and a total of 12 single spores were isolated from the nine strains of . After the MAT idiomorph was confirmed by PCR, 36 mating combinations were performed with six single spores with and the others with . Eight mating combinations were successfully mated, producing stroma with perithecia. Cordycepin content analysis of all strains by high-performance liquid chromatography revealed that the KASP4-bred strain produced the maximum cordycepin among all strains, regardless of the medium and stroma parts. Finally, universal rice primer-PCR was performed to demonstrate that the bred strains were genetically different from the parental strains and new strains. These results may be related to the recombination of genes during mating. The newly produced strains can be used to meet the industrial demand for cordycepin. In addition, breeding through mating suggests the possibility of producing numerous cordycepin-producing strains.

摘要

冬虫夏草属子囊菌门,是一种被称为冬虫夏草的蘑菇,因其含有包括虫草素在内的高含量生物活性物质以及人工栽培潜力而具有商业价值。虫草素(3'-脱氧腺苷)与冬虫夏草的药理作用高度相关。冬虫夏草是异宗配合的,即两个交配型位点,特异型和,分别存在于两个不同的孢子中。在本研究中,将9株冬虫夏草菌株相互交配,以制备出比亲本菌株产生更多虫草素的新培育菌株。通过比较内部转录间隔区序列鉴定出9株冬虫夏草菌株,并从这9株冬虫夏草菌株中总共分离出12个单孢子。通过PCR确认交配型特异型后,用6个单孢子与特异型进行36种交配组合,其余与特异型进行交配组合。成功交配了8种组合,产生了带有子囊壳的子座。通过高效液相色谱法对所有菌株的虫草素含量进行分析,结果表明,无论培养基和子座部分如何,KASP4培育的菌株在所有菌株中产生的虫草素最多。最后,进行通用水稻引物-PCR以证明培育的菌株在基因上与亲本菌株不同,是新的冬虫夏草菌株。这些结果可能与交配过程中的基因重组有关。新产生的菌株可用于满足工业对虫草素的需求。此外,通过交配育种表明有可能产生大量产虫草素的冬虫夏草菌株。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验