Crnogaj Martina, Cerón José Joaquin, Šmit Iva, Kiš Ivana, Gotić Jelena, Brkljačić Mirna, Matijatko Vesna, Rubio Camila Peres, Kučer Nada, Mrljak Vladimir
Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, University of Murcia, 30100 Espinardo, Murcia, Spain.
BMC Vet Res. 2017 Apr 24;13(1):114. doi: 10.1186/s12917-017-1020-9.
Canine babesiosis is caused by species of the Babesia genus and has become an emerging disease worldwide. To the authors' knowledge there are no reports in which antioxidants have been analyzed in different presentations of canine babesiosis or in which the prognostic value of antioxidants has been studied. The aim of this study was to evaluate whether oxidative stress could be related to the severity and outcome of canine babesiosis. For this purpose a profile consisting of four antioxidant biomarkers (superoxide dismutase - SOD, glutathione peroxidase - GPx, catalase, total antioxidant status - TAS) and malondialdehyde - MDA as an oxidant biomarker (previously evaluated, here studied for comparative purposes) were evaluated in dogs with canine babesiosis of different clinical severity and outcomes.
The study was conducted with a sample of 40 dogs suffering from babesiosis (further divided into uncomplicated, one complication and multiple organ dysfunction syndrome - MODS group) and 30 healthy dogs (control group). Additionally, the babesiosis group was divided according to the anaemia into non-anaemic, mildly anaemic, moderately anaemic and severely anaemic dogs. The results of our study showed significantly decreased SOD, catalase and TAS values in diseased dogs compared to controls, while there were no significant differences in GPx between these groups. Dogs that developed MODS showed lower activities of SOD and GPx and higher MDA values compared to dogs with uncomplicated babesiosis as well as with dogs that developed one complication. Superoxide dismutase, catalase and GPx were negatively correlated whereas MDA was positively correlated with the lethal outcome of the disease. Furthermore, this study detected more pronounced decrease in antioxidant biomarkers (SOD, GPx and catalase) in dogs with moderate anaemia compared to those with mild anaemia.
The results of this study showed changes in biomarkers related to the antioxidant status of dogs naturally infected with B. canis canis. These biomarkers could be used as indicators of disease severity and outcome in dogs suffering from babesiosis.
犬巴贝斯虫病由巴贝斯属的物种引起，已成为一种在全球范围内出现的疾病。据作者所知，尚无关于在犬巴贝斯虫病的不同表现形式中分析抗氧化剂或研究抗氧化剂预后价值的报道。本研究的目的是评估氧化应激是否可能与犬巴贝斯虫病的严重程度和预后相关。为此，在患有不同临床严重程度和预后的犬巴贝斯虫病的犬只中，评估了由四种抗氧化生物标志物（超氧化物歧化酶 - SOD、谷胱甘肽过氧化物酶 - GPx、过氧化氢酶、总抗氧化状态 - TAS）和作为氧化生物标志物的丙二醛 - MDA（此前已评估，此处为比较目的而研究）组成的指标。
该研究以40只患有巴贝斯虫病的犬（进一步分为无并发症、有一处并发症和多器官功能障碍综合征 - MODS组）和30只健康犬（对照组）为样本进行。此外，巴贝斯虫病组根据贫血情况分为非贫血、轻度贫血、中度贫血和重度贫血犬。我们的研究结果显示，与对照组相比，患病犬的SOD、过氧化氢酶和TAS值显著降低，而这些组之间的GPx没有显著差异。与无并发症的巴贝斯虫病犬以及出现一处并发症的犬相比，发生MODS的犬SOD和GPx活性较低，MDA值较高。超氧化物歧化酶、过氧化氢酶和GPx与疾病的致死结局呈负相关，而MDA与疾病的致死结局呈正相关。此外，本研究发现，与轻度贫血犬相比，中度贫血犬的抗氧化生物标志物（SOD、GPx和过氧化氢酶）下降更为明显。
本研究结果显示，自然感染犬巴贝斯虫的犬的抗氧化状态相关生物标志物发生了变化。这些生物标志物可作为患有巴贝斯虫病犬的疾病严重程度和预后指标。
