Ładno W, Gawlak M, Szulczyk P, Nurowska E
Laboratory of Physiology and Pathophysiology, Medical University of Warsaw, Centre of Preclinical Research and Technology, Banacha 1b, Warsaw, Poland.
Laboratory of Physiology and Pathophysiology, Medical University of Warsaw, Centre of Preclinical Research and Technology, Banacha 1b, Warsaw, Poland. Electronic address: http://zfc.wum.edu.pl/.
Brain Res. 2017 Jun 15;1665:95-104. doi: 10.1016/j.brainres.2017.04.009. Epub 2017 Apr 21.
TREK-2-like channels were identified on the basis of electrophysiological and pharmacological tests performed on freshly isolated and enzymatically/mechanically dispersed pyramidal neurons of the rat medial prefrontal cortex (mPFC). Single-channel currents were recorded in cell-attached configuration and the impact of adrenergic receptors (α, α, β) stimulation on spontaneously appearing TREK-2-like channel activity was tested. The obtained results indicate that noradrenaline decreases the mean open probability of TREK-2-like channel currents by activation of β but not of α- and α-adrenergic receptors. Mean open time and channel conductance were not affected. The system of intracellular signaling pathways depends on the activation of protein kinase A. We also show that adrenergic control of TREK-2-like channel currents by adrenergic receptors was similar in pyramidal neurons isolated from young, adolescent, and adult rats. Immunofluorescent confocal scans of mPFC slices confirmed the presence of the TREK-2 protein, which was abundant in layer V pyramidal neurons. The role of TREK-2-like channel control by adrenergic receptors is discussed.
基于对大鼠内侧前额叶皮质（mPFC）新鲜分离及酶解/机械分散的锥体神经元进行的电生理和药理学测试，鉴定出了类TREK-2通道。在细胞贴附模式下记录单通道电流，并测试肾上腺素能受体（α、α、β）刺激对自发出现的类TREK-2通道活性的影响。所得结果表明，去甲肾上腺素通过激活β肾上腺素能受体而非α和α肾上腺素能受体来降低类TREK-2通道电流的平均开放概率。平均开放时间和通道电导未受影响。细胞内信号通路系统依赖于蛋白激酶A的激活。我们还表明，在从幼年、青春期和成年大鼠分离的锥体神经元中，肾上腺素能受体对类TREK-2通道电流的肾上腺素能控制是相似的。mPFC切片的免疫荧光共聚焦扫描证实了TREK-2蛋白的存在，该蛋白在V层锥体神经元中大量存在。本文讨论了肾上腺素能受体对类TREK-2通道控制的作用。
