Grzelka Katarzyna, Gawlak Maciej, Czarzasta Katarzyna, Szulczyk Paweł
Laboratory of Physiology and Pathophysiology, Centre for Preclinical Research and Technology, Medical University of Warsaw, Banacha 1B, 02-097, Warsaw, Poland.
Laboratory of Experimental and Clinical Physiology, Centre for Preclinical Research and Technology, Medical University of Warsaw, Banacha 1B, 02-097, Warsaw, Poland.
Neurosci Lett. 2019 Apr 17;698:192-197. doi: 10.1016/j.neulet.2019.01.022. Epub 2019 Jan 11.
While the expression of β-adrenergic receptors is firmly established in adipose, kidney and heart tissue, their expression and function in the brain remains unclear despite their potential role in depression and stress-related disorders. This study aimed to investigate the expression of β-adrenoreceptors and their involvement in the mechanism controlling the resting holding current in layer V medial prefrontal cortex (mPFC) pyramidal neurons in young rats. Applications of the selective β-adrenergic receptor agonists BRL 37344 and SR 58611 A evoked inward currents in the tested neurons. The inward currents evoked by BRL 37344 or noradrenaline (a nonselective physiological adrenergic receptor agonist) were prevented or decreased, respectively, by the selective β-receptor antagonist L-748,337. Western blot and fluorescence immunohistochemistry analyses revealed β-adrenergic receptor protein expression in the mPFC. Thus, based on the results obtained here, functional β-adrenergic receptors are expressed in layer V mPFC pyramidal neurons and their activation evokes inward currents.
虽然β-肾上腺素能受体在脂肪组织、肾脏和心脏组织中的表达已得到确证，但尽管它们在抑郁症和应激相关疾病中可能发挥作用，其在大脑中的表达和功能仍不清楚。本研究旨在调查幼鼠内侧前额叶皮层（mPFC）第V层锥体神经元中β-肾上腺素能受体的表达及其在控制静息保持电流机制中的作用。选择性β-肾上腺素能受体激动剂BRL 37344和SR 58611 A的应用在受试神经元中诱发内向电流。选择性β-受体拮抗剂L-748,337分别阻断或降低了BRL 37344或去甲肾上腺素（一种非选择性生理性肾上腺素能受体激动剂）诱发的内向电流。蛋白质免疫印迹和荧光免疫组织化学分析显示mPFC中存在β-肾上腺素能受体蛋白表达。因此，基于此处获得的结果，功能性β-肾上腺素能受体在mPFC第V层锥体神经元中表达，其激活可诱发内向电流。
