禾本科植物中EST-SSR标记的开发与特性分析
Development and characterization of EST-SSR markers in (Poaceae).
作者信息
Ren Jing, Su Zhi-Zhen, Dang Zhen-Hua, Ding Yu, Wang Pei-Xuan, Niu Jian-Ming
机构信息
School of Ecology and Environmental Sciences, Inner Mongolia University, Hohhot, People's Republic of China.
Sino-US Center for Conservation, Energy, and Sustainability Science, Inner Mongolia University, Hohhot, People's Republic of China.
出版信息
Appl Plant Sci. 2017 Apr 12;5(4). doi: 10.3732/apps.1600157. eCollection 2017 Apr.
PREMISE OF THE STUDY
(Poaceae) is one of the dominant species of the desert steppe in the eastern Eurasian grasslands. Simple sequence repeat (SSR) markers were developed for use in genetic diversity studies of this species.
METHODS AND RESULTS
A total of 1954 potentially polymorphic loci were obtained by comparing transcriptome data of eight different individuals. We selected 81 loci to verify polymorphism and 63 loci amplified, of which 21 loci exhibited polymorphism. The number of alleles per locus varied from two to 24, the observed heterozygosity ranged from 0.083 to 0.958, and the expected heterozygosity ranged from 0.396 to 0.738.
CONCLUSIONS
These newly identified SSR loci can be used for population genetic and landscape genetic studies of . In addition, 14 loci also amplified in six related species (, , , , , and ).
研究前提
（禾本科）是欧亚大陆东部草原荒漠草原的优势物种之一。开发了简单序列重复（SSR）标记用于该物种的遗传多样性研究。
方法与结果
通过比较八个不同个体的转录组数据，共获得1954个潜在多态性位点。我们选择81个位点验证多态性，63个位点扩增，其中21个位点表现出多态性。每个位点的等位基因数从2到24不等，观察到的杂合度范围为0.083至0.958，预期杂合度范围为0.396至0.738。
结论
这些新鉴定的SSR位点可用于该物种的群体遗传学和景观遗传学研究。此外，14个位点在六个相关物种（、、、、、和）中也能扩增。
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