Guangdong Key Laboratory of Plant Resources and State Key Laboratory of Biocontrol, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.
Appl Plant Sci. 2013 Jan 2;1(1). doi: 10.3732/apps.1200211. eCollection 2013 Jan.
Spartina alterniflora is one of the nine most notoriously invasive plants in China. Microsatellite markers were developed for this species to investigate its invasiveness and genetic diversity. •
Fifteen polymorphic and seven monomorphic simple sequence repeat (SSR) markers derived from expressed sequence tags (ESTs) were identified and screened in 60 samples of S. alterniflora. The number of alleles per polymorphic locus ranged from two to eight, with an average of 3.8 alleles per polymorphic locus. The expected heterozygosity and observed heterozygosity based on seven disomic loci ranged from 0.27 to 0.46 and 0.21 to 0.51, respectively. The average Shannon index ranged from 0.26 to 0.94 in eight nondisomic loci. •
The SSR markers described here may be useful for further investigation of population genetics and invasion dynamics of S. alterniflora.
互花米草是中国最具恶名的入侵植物之一。本研究开发了微卫星标记来研究其入侵性和遗传多样性。
从表达序列标签(ESTs)中鉴定并筛选了 60 个互花米草样本中的 15 个多态性和 7 个单态性简单重复序列(SSR)标记。每个多态性位点的等位基因数从两个到八个不等,平均每个多态性位点有 3.8 个等位基因。基于七个二倍体位点的预期杂合度和观察杂合度分别为 0.27 至 0.46 和 0.21 至 0.51。在八个非二倍体位点中,平均香农指数范围为 0.26 至 0.94。
本文描述的 SSR 标记可能有助于进一步研究互花米草的种群遗传学和入侵动态。