使用F-FBPA PET估算组织中绝对硼浓度的实用计算方法。
Practical calculation method to estimate the absolute boron concentration in tissues using F-FBPA PET.
作者信息
Watabe Tadashi, Hanaoka Kohei, Naka Sadahiro, Kanai Yasukazu, Ikeda Hayato, Aoki Masanao, Shimosegawa Eku, Kirihata Mitsunori, Hatazawa Jun
机构信息
Department of Nuclear Medicine and Tracer Kinetics, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka, 565-0871, Japan.
Medical Imaging Center for Translational Research, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
出版信息
Ann Nucl Med. 2017 Jul;31(6):481-485. doi: 10.1007/s12149-017-1172-5. Epub 2017 Apr 24.
PURPOSE
The purpose of this study was to establish a practical method to estimate the absolute boron concentrations in the tissues based on the standardized uptake values (SUVs) after administration of 4-borono-phenylalanine (BPA) using 4-borono-2-F-fluoro-phenylalanine (F-FBPA) PET.
METHODS
Rat xenograft models of C6 glioma (n = 7, body weight 241 ± 28.0 g) were used for the study. PET was performed 60 min after intravenous injection of F-FBPA (30.5 ± 0.7 MBq). After the PET scanning, BPA-fructose (167.3 ± 18.65 mg/kg) was administered by slow intravenous injection to the same subjects. The rats were killed 60 min after the BPA injection and tissue samples were collected from the major organs and tumors. The absolute boron concentrations (unit: ppm) in the samples were measured by inductively coupled plasma optical emission spectrometry (ICP-OES). The boron concentrations in the tissues/tumors were also estimated from the F-FBPA PET images using the following formula: estimated absolute boron concentration (ppm) = 0.0478 × [BPA dose (mg/kg)] × SUV. The measured absolute boron concentrations (mBC) by ICP-OES and the estimated boron concentrations (eBC) from the PET images were compared.
RESULTS
The percent difference between the mBC and eBC calculated based on the SUV was -5.2 ± 21.1% for the blood, -9.4 ± 22.3% for the brain, 1.6 ± 21.3% for the liver, -14.3 ± 16.8% for the spleen, -9.5 ± 27.5% for the pancreas, and 3.4 ± 43.2% for the tumor. Relatively large underestimation was observed for the lung (-48.4 ± 16.2%), small intestine (-37.8 ± 19.3%) and large intestine (-33.9 ± 11.0%), due to the partial volume effect arising from the air or feces contained in these organs. In contrast, relatively large overestimation was observed for the kidney (34.3 ± 29.3%), due to the influence of the high uptake in urine.
CONCLUSIONS
The absolute boron concentrations in tissues/tumors can be estimated from the SUVs on F-FBPA PET using a practical formula. Caution must be exercised in interpreting the estimated boron concentrations in the lung, small intestine and large intestine, to prevent the adverse effects of overexposure, which could occur due to underestimation by partial volume effect using PET.
目的
本研究的目的是建立一种实用方法，基于使用4-硼-2-F-氟苯丙氨酸（F-FBPA）PET在给予4-硼苯丙氨酸（BPA）后获得的标准化摄取值（SUV）来估计组织中的绝对硼浓度。
方法
使用C6胶质瘤大鼠异种移植模型（n = 7，体重241±28.0 g）进行研究。静脉注射F-FBPA（30.5±0.7 MBq）后60分钟进行PET检查。PET扫描后，通过缓慢静脉注射向同一组受试者给予BPA-果糖（167.3±18.65 mg/kg）。BPA注射后60分钟处死大鼠，从主要器官和肿瘤中采集组织样本。通过电感耦合等离子体发射光谱法（ICP-OES）测量样本中的绝对硼浓度（单位：ppm）。还使用以下公式从F-FBPA PET图像估计组织/肿瘤中的硼浓度：估计的绝对硼浓度（ppm）= 0.0478×[BPA剂量（mg/kg）]×SUV。比较通过ICP-OES测量的绝对硼浓度（mBC）和从PET图像估计的硼浓度（eBC）。
结果
基于SUV计算的mBC和eBC之间的百分比差异，血液为-5.2±21.1%，脑为-9.4±22.3%，肝脏为1.6±21.3%，脾脏为-14.3±16.8%，胰腺为-9.5±27.5%，肿瘤为3.4±43.2%。对于肺（-48.4±16.2%）、小肠（-37.8±19.3%）和大肠（-33.9±11.0%）观察到相对较大的低估，这是由于这些器官中所含空气或粪便引起的部分容积效应。相反，由于尿液中高摄取的影响，肾脏观察到相对较大的高估（34.3±29.3%）。
结论
可以使用实用公式从F-FBPA PET上的SUV估计组织/肿瘤中的绝对硼浓度。在解释肺、小肠和大肠中估计的硼浓度时必须谨慎，以防止因使用PET的部分容积效应低估而可能发生的过度暴露的不良影响。
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