Wuhrmann P, Ineichen H, Riesen-Willi U, Lezzi M
Proc Natl Acad Sci U S A. 1979 Feb;76(2):806-8. doi: 10.1073/pnas.76.2.806.
Changes in nuclear K+ electrochemical activity and total nuclear K+ content in salivary glands of Chironomus tentans were measured with ion-selective microelectrodes based on valinomycin and with flameless atomic absorption spectrometry, respectively. The K+ activity increased by a factor of 2.6 and the total K+, by a factor of 1.5 as oligopausing larvae developed into prepupae. The extent of decondensation (puffing) of K+-sensitive regions in the polytene chromosomes underwent a parallel increase during this developmental event. In vitro culture of glands from oligopausing larvae resulted in similar changes with respect to nuclear K+ activity and puffing.
分别使用基于缬氨霉素的离子选择性微电极和无火焰原子吸收光谱法,测定了摇蚊唾液腺中核钾离子电化学活性和核钾离子总含量的变化。随着寡停育幼虫发育成预蛹,钾离子活性增加了2.6倍,总钾离子增加了1.5倍。在此发育过程中,多线染色体中钾离子敏感区域的解聚(膨松)程度也随之平行增加。对寡停育幼虫的腺体进行体外培养,在核钾离子活性和膨松方面也产生了类似变化。